Harichane Yassine, Dimitrova-Nakov Sasha, Marchadier Arnaud, Collet Corinne, Baudry Anne, Vidal Catherine, Kamoun-Goldrat Agnès, Kellermann Odile, Goldberg Michel
INSERM, UMR-S 747, F-75006, Paris, France.
Eur J Oral Sci. 2011 Dec;119 Suppl 1:177-84. doi: 10.1111/j.1600-0722.2011.00908.x.
The role of the serotonin 2B receptor (5-HT(2B) R) in enamel formation and mineralization was explored in adult 5HT(2B) R knockout (KO) mice compared with wild-type (WT) mice. In the molar, quantitative data obtained by micro-computed tomography imaging showed that the overall volume of the enamel layer was firmly reduced in KO mice. Defective mineralization was ascertained by energy-dispersive X-ray microanalysis. We also observed, using scanning electron microscopy, that parazones in the KO mice included two or three helicoidally twisted rods within Hunter-Schreger bands, instead of a single rod, as found in the WT mice. Minor disturbances were also detected in the incisors of KO mice. Structural modifications, thinner enamel crystallites, and porosities observed in KO mice indicate that the 5-HT(2B) R-mediated signaling pathways as part of the enamel formation process. These data provide a basis for evaluating the role of 5-HT(2B) R in ameloblast functions. Defects observed in the mineralization and structure of enamel in KO mice highlight that the 5-HT(2B) R interferes with the mechanisms directing amelogenesis.
与野生型（WT）小鼠相比，在成年5-羟色胺2B受体（5-HT(2B)R）基因敲除（KO）小鼠中探索了5-HT(2B)R在牙釉质形成和矿化中的作用。在磨牙中，通过微计算机断层扫描成像获得的定量数据显示，KO小鼠牙釉质层的总体积明显减少。通过能量色散X射线微分析确定矿化存在缺陷。我们还使用扫描电子显微镜观察到，KO小鼠的釉板在亨特-施雷格板内包含两到三根螺旋扭曲的釉柱，而不是像WT小鼠那样只有一根釉柱。在KO小鼠的门牙中也检测到了轻微的紊乱。在KO小鼠中观察到的结构改变、牙釉质微晶变薄和孔隙率表明，5-HT(2B)R介导的信号通路是牙釉质形成过程的一部分。这些数据为评估5-HT(2B)R在成釉细胞功能中的作用提供了依据。在KO小鼠牙釉质矿化和结构中观察到的缺陷突出表明，5-HT(2B)R干扰了指导釉质形成的机制。
