Tamburstuen Margareth V, Snead Malcolm L, Reseland Janne E, Paine Michael L, Lyngstadaas Staale P
Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo (UiO), Oslo, Norway.
Eur J Oral Sci. 2011 Dec;119 Suppl 1(Suppl 1):286-92. doi: 10.1111/j.1600-0722.2011.00910.x.
Ameloblastin (AMBN) was originally described as a tooth-specific extracellular matrix protein, but current data have shown that AMBN is present in many different tissues of mesenchymal origin. The identification of regulatory elements in the promoter region of the Ambn gene would assist in identifying potential mesenchymal-specific transcriptional factors. In this study we subcloned a 3,788-bp region upstream (and a 54-bp region downstream) of the mouse Ambn transcriptional start site into a LacZ reporter construct and called this construct 3788-Ambn-lacZ. In silico analysis of the 3,788-bp Ambn promoter region identified 50 potential cis-regulatory elements, 29 of which are known to be functional in cell populations of mesenchymal origin. The reporter construct was activated in transfected bone marrow cells, and the promoter activity was induced in cell cultures following addition of recombinant AMBN, interferon-γ, serotonin, or dexamethasone. We discuss the relative significance of the potential cis-acting gene-regulatory elements of Ambn in relation to bone morphogenesis. Knowledge of Ambn gene-regulatory elements will be of importance when developing strategies for bone repair and replacement in a clinical surgical setting.
成釉蛋白（AMBN）最初被描述为一种牙齿特异性细胞外基质蛋白，但目前的数据表明，AMBN存在于许多间充质来源的不同组织中。鉴定Ambn基因启动子区域的调控元件将有助于识别潜在的间充质特异性转录因子。在本研究中，我们将小鼠Ambn转录起始位点上游3788 bp区域（以及下游54 bp区域）亚克隆到LacZ报告基因构建体中，并将该构建体命名为3788-Ambn-lacZ。对3788 bp的Ambn启动子区域进行的计算机分析确定了50个潜在的顺式调控元件，其中29个已知在间充质来源的细胞群体中具有功能。报告基因构建体在转染的骨髓细胞中被激活，并且在添加重组AMBN、干扰素-γ、血清素或地塞米松后，细胞培养物中的启动子活性被诱导。我们讨论了Ambn潜在的顺式作用基因调控元件与骨形态发生相关的相对重要性。在临床手术环境中制定骨修复和置换策略时，了解Ambn基因调控元件将具有重要意义。
