通过一种基于 ATP 的氨基酸添加策略增强 S-腺苷甲硫氨酸和谷胱甘肽的共生产。
Enhanced co-production of S-adenosylmethionine and glutathione by an ATP-oriented amino acid addition strategy.
机构信息
School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China.
出版信息
Bioresour Technol. 2012 Mar;107:19-24. doi: 10.1016/j.biortech.2011.12.030. Epub 2011 Dec 30.
Effects of amino acids on co-production of S-adenosylmethionine (SAM) and glutathione (GSH) with Candida utilis CCTCC M 209298 were investigated. Both methionine and cysteine showed positive effects on improving intracellular SAM and GSH content simultaneously. Batch fermentative co-production of SAM and GSH was conducted in a 5L stirred fermentor, where the co-production of SAM and GSH was enhanced by 71.3% and 71.5% with 6g/L methionine and 6 mmol/L cysteine, respectively. Based on these results together with intracellular ATP levels during batch cultivation of C. utilis CCTCC M 209298, a two-stage amino acid addition strategy was developed by adding 6g/L methionine at 0 h and 6 mmol/L cysteine at 21 h. Under this ATP-oriented strategy, SAM and GSH biosynthesis was further promoted, and the co-production of SAM and GSH reached 669.3mg/L, which was increased by 124.6% compared to that without amino acid addition.
考察了氨基酸对产 S-腺苷甲硫氨酸（SAM）和谷胱甘肽（GSH）与 Candida utilis CCTCC M 209298 共生产的影响。蛋氨酸和半胱氨酸均对同时提高细胞内 SAM 和 GSH 含量有积极影响。在 5L 搅拌发酵罐中进行了 SAM 和 GSH 的分批发酵共生产，分别添加 6g/L 蛋氨酸和 6mmol/L 半胱氨酸可使 SAM 和 GSH 的共生产分别提高 71.3%和 71.5%。基于这些结果以及在 C. utilis CCTCC M 209298 的分批培养过程中细胞内 ATP 水平，开发了一种两阶段氨基酸添加策略，即在 0 小时添加 6g/L 蛋氨酸，在 21 小时添加 6mmol/L 半胱氨酸。在这种以 ATP 为导向的策略下，SAM 和 GSH 的生物合成进一步得到促进，SAM 和 GSH 的共生产达到 669.3mg/L，与不添加氨基酸相比，增加了 124.6%。
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