Yin Sheng, Yang Dongmei, Zhu Yiying, Huang Baozhu
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing, China.
Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing, China.
Front Microbiol. 2022 Jun 14;13:921540. doi: 10.3389/fmicb.2022.921540. eCollection 2022.
Amino acid metabolism could exert regulatory effects on pigments (MPs) biosynthesis. In this work, MPs biosynthesis regulated by methionine and S-adenosylmethionine (SAM) was investigated in RP2. The results indicated that the addition of methionine in fermentation significantly reduced MPs production by 60-70%, and it induced a higher expression of SAM synthetase Mon2A2272 and consequently led to SAM accumulation. However, the addition of SAM in fermentation promoted MPs production by a maximum of 35%, while over-expression of the gene led to a decrease in MPs yield, suggesting that SAM synthetase and SAM were likely to play different regulatory roles in MPs biosynthesis. Furthermore, the gene transcription profile indicated that SAM synthetase expression led to a higher expression of the transcriptional regulatory protein of the MPs biosynthesis gene cluster, while the addition of SAM gave rise to a higher expression of MPs biosynthesis activator and the global regulator LaeA, which probably accounted for changes in MPs production and the mycelium colony morphology of RP2 triggered by methionine and SAM. This work proposed a possible regulation mechanism of MPs biosynthesis by SAM metabolism from methionine. The findings provided a new perspective for a deep understanding of MPs biosynthesis regulation in
氨基酸代谢可能对色素（MPs）的生物合成产生调控作用。在本研究中，对RP2中由甲硫氨酸和S-腺苷甲硫氨酸（SAM）调控的MPs生物合成进行了研究。结果表明，在发酵过程中添加甲硫氨酸显著降低了MPs产量，降幅达60 - 70%，并诱导了SAM合成酶Mon2A2272的更高表达，进而导致SAM积累。然而，在发酵过程中添加SAM最多可使MPs产量提高35%，而该基因的过表达却导致MPs产量下降，这表明SAM合成酶和SAM在MPs生物合成中可能发挥不同的调控作用。此外，基因转录谱表明，SAM合成酶的表达导致MPs生物合成基因簇转录调控蛋白的更高表达，而添加SAM则使MPs生物合成激活剂和全局调控因子LaeA的表达更高，这可能解释了由甲硫氨酸和SAM引发的RP2的MPs产量及菌丝菌落形态的变化。本研究提出了一种由甲硫氨酸的SAM代谢对MPs生物合成的可能调控机制。这些发现为深入理解……中的MPs生物合成调控提供了新的视角
