Ramírez M, Arechaga G, Garcia S, Sanchez B, Lardelli P, de Gandarias J M
Department of Physiology, Faculty of Medicine, University of the Basque Country, Leioa, Vizcaya, Spain.
Brain Res. 1990 Jul 2;522(1):165-7. doi: 10.1016/0006-8993(90)91595-8.
The levels of soluble and membrane-bound aminopeptidase activities were assayed in subcellular fractions from young (4 weeks old) and adult (20 weeks old) rat brains, using Asp-2-naphthylamide as substrate. The young rats showed the highest soluble and membrane-bound levels of activity in the microsomal fraction but no differences among fractions were found at 20 weeks of age. The membrane-bound activity was significantly higher than the soluble one in all subcellular fractions of young rats. Soluble activity of the homogenate and the mitochondrial fraction was significantly increased in adult animals when compared to that of younger ones.
以天冬氨酸 -2-萘酰胺为底物，测定了4周龄幼龄和20周龄成年大鼠脑亚细胞组分中可溶性和膜结合型氨肽酶的活性水平。幼龄大鼠在微粒体组分中可溶性和膜结合型活性水平最高，但在20周龄时各组分间未发现差异。在幼龄大鼠的所有亚细胞组分中，膜结合型活性均显著高于可溶性活性。与幼龄动物相比，成年动物匀浆和线粒体组分的可溶性活性显著增加。
