Department of Pharmacology, Doshisha Women's College of Liberal Arts, 97-1 Minamihokodate, Kodo, Kyotanabe, Kyoto 610-0395, Japan.
J Neuroimmunol. 2012 Mar;244(1-2):16-22. doi: 10.1016/j.jneuroim.2011.12.022. Epub 2012 Jan 14.
Immunological stimulation of T cells by phytohemagglutinin (PHA) enhances the synthesis and release of acetylcholine (ACh), suggesting a role for the lymphocytic cholinergic system in the regulation of immune function. In the present study, we used two human leukemic T cell lines as models to investigate whether mediatophore, a homooligomer of a 16-kDa subunit homologous to the proteolipid subunit c of vacuolar H(+)-ATPase (V-ATPase), is involved in mediating ACh release from T cells. Immunohistochemical analysis revealed the presence of mediatophore in the cytoplasm and on the plasma membrane of both T cell lines. Mediatophore gene expression was up-regulated by immunological T cell activation by PHA. Transfection of anti-mediatophore small interference RNA down-regulated mediatophore gene expression and significantly reduced ACh release. These results suggest that T cells express mediatophore, which then plays a key role in mediating ACh release, and that mediatophore expression is regulated by immunological stimulation.
植物血球凝集素（PHA）对 T 细胞的免疫刺激增强了乙酰胆碱（ACh）的合成和释放，这表明淋巴细胞胆碱能系统在调节免疫功能方面发挥作用。在本研究中，我们使用两种人类白血病 T 细胞系作为模型，研究介质体（一种与液泡 H(+)-ATP 酶（V-ATPase）的蛋白脂质亚基 c 同源的 16kDa 亚基的同源寡聚物）是否参与介导 T 细胞中 ACh 的释放。免疫组织化学分析显示两种 T 细胞系的细胞质和质膜上均存在介质体。PHA 诱导的免疫 T 细胞激活上调了介质体基因的表达。转染抗介质体小干扰 RNA 下调了介质体基因的表达并显著减少了 ACh 的释放。这些结果表明 T 细胞表达介质体，后者在介导 ACh 释放中起关键作用，并且介质体的表达受免疫刺激调节。
