[Effect of transfection of enhanced green fluorescent protein gene on neuron-like differentiation of rat mesenchymal stem cells].
作者信息
Li Yawei, Wang Bing, Lu Guohua, Liu Weidong, Li Lei
机构信息
Department of Spinal Surgery, Second Xiangya Hospital, Central South University, Changsha, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011 Dec;36(12):1183-8. doi: 10.3969/j.issn.1672-7347.2011.12.010.
OBJECTIVE
To determine the effect of transfection of enhanced green fluorescent protein (EGFP) on neuron-like differentiation of rat mesenchymal stem cells (rMSC) with plasmid vector.
METHODS
In vitro cultured rMSC was transfected with plasmid vector containing EGFP, and its surface marker and differentiation neuron-like cells were detected. Results There was no significant difference in the morphology and surface markers between the EGFP-rMSC and rMSC. The cell surface markers of EGFP-rMSC including expression of CD44(+),CD11b (-) and CD45(-) remained similar to those of rMSC. EGFP-rMSC presented the differentiated potential to neuron-like cells. There was no statistical difference in the positive ratio of neuron-like differentiation between the EGFP-rMSC and rMSC (P> 0.05).
CONCLUSION
EGFP does not affect the neuron-like differentiation potential of rMSC, and can be used as the trace marker in the study of differentiation potential of rMSC.
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