开发一种检测丁型肝炎病毒抗体的方法，以研究中国乙型肝炎病毒感染者中丁型肝炎病毒的流行情况。

Development of a hepatitis delta virus antibody assay for study of the prevalence of HDV among individuals infected with hepatitis B virus in China.

机构信息

State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping District, Beijing, China.

出版信息

J Med Virol. 2012 Mar;84(3):445-9. doi: 10.1002/jmv.23212.

DOI:10.1002/jmv.23212

PMID:22246830

Abstract

Co-infection with hepatitis delta virus (HDV) and hepatitis B virus (HBV) has been shown to be associated with a more severe form of acute and chronic hepatitis. Cloning and expression of recombinant HDV antigen (rHDAg) in Escherichiacoli are described. Using purified rHDAg, a cost-effective indirect anti-HDV enzyme-linked immunosorbent assay (ELISA) kit was developed. Direct comparison of 15 known HDV-positive sera and 15 HDV-negative sera showed concordance agreement between the new assay kit and the Abbott Murex Anti-Delta (total) kit. In addition, 1,486 hepatitis B surface antigen (HBsAg) positive blood samples collected from various areas of China were tested using this indirect anti-HDV ELISA. It was found that 1.2% (95% CI: 0.7-1.9%) of the samples were anti-HDAg positive. It is suggested that the prevalence of HDV and HBV co-infection in China is relatively low.

摘要

已证实，乙型肝炎病毒（HBV）和δ型肝炎病毒（HDV）共同感染与更严重形式的急性和慢性肝炎相关。本文描述了大肠杆菌中重组 HDV 抗原（rHDAg）的克隆和表达。使用纯化的 rHDAg，开发了一种具有成本效益的间接抗-HDV 酶联免疫吸附测定（ELISA）试剂盒。新试剂盒与 Abbott Murex Anti-Delta（总）试剂盒对 15 份已知的 HDV 阳性血清和 15 份 HDV 阴性血清的直接比较显示出一致性。此外，用这种间接抗-HDV ELISA 检测了来自中国不同地区的 1486 份乙型肝炎表面抗原（HBsAg）阳性的血液样本。结果发现，1.2%（95%CI：0.7-1.9%）的样本抗-HDAg 阳性。这表明中国 HDV 和 HBV 共同感染的流行率相对较低。