State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping District, Beijing, China.
J Med Virol. 2012 Mar;84(3):445-9. doi: 10.1002/jmv.23212.
Co-infection with hepatitis delta virus (HDV) and hepatitis B virus (HBV) has been shown to be associated with a more severe form of acute and chronic hepatitis. Cloning and expression of recombinant HDV antigen (rHDAg) in Escherichiacoli are described. Using purified rHDAg, a cost-effective indirect anti-HDV enzyme-linked immunosorbent assay (ELISA) kit was developed. Direct comparison of 15 known HDV-positive sera and 15 HDV-negative sera showed concordance agreement between the new assay kit and the Abbott Murex Anti-Delta (total) kit. In addition, 1,486 hepatitis B surface antigen (HBsAg) positive blood samples collected from various areas of China were tested using this indirect anti-HDV ELISA. It was found that 1.2% (95% CI: 0.7-1.9%) of the samples were anti-HDAg positive. It is suggested that the prevalence of HDV and HBV co-infection in China is relatively low.
已证实，乙型肝炎病毒（HBV）和δ型肝炎病毒（HDV）共同感染与更严重形式的急性和慢性肝炎相关。本文描述了大肠杆菌中重组 HDV 抗原（rHDAg）的克隆和表达。使用纯化的 rHDAg，开发了一种具有成本效益的间接抗-HDV 酶联免疫吸附测定（ELISA）试剂盒。新试剂盒与 Abbott Murex Anti-Delta（总）试剂盒对 15 份已知的 HDV 阳性血清和 15 份 HDV 阴性血清的直接比较显示出一致性。此外，用这种间接抗-HDV ELISA 检测了来自中国不同地区的 1486 份乙型肝炎表面抗原（HBsAg）阳性的血液样本。结果发现，1.2%（95%CI：0.7-1.9%）的样本抗-HDAg 阳性。这表明中国 HDV 和 HBV 共同感染的流行率相对较低。
