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从刺猬（Erinaceus europaeus）中克隆、表达和功能表征增殖诱导配体（APRIL）。

Gene cloning, expression and functional characterization of a proliferation-inducing ligand (APRIL) from hedgehog (Erinaceus europaeus).

机构信息

Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210046, China.

出版信息

Mol Biol Rep. 2012 May;39(5):6123-32. doi: 10.1007/s11033-011-1428-4. Epub 2012 Jan 14.

DOI:10.1007/s11033-011-1428-4
PMID:22246941
Abstract

Here we describe the identification of the hedgehog Erinaceus europaeus homologue of a proliferation-inducing ligand (APRIL) of the TNF family (designated heAPRIL). Hedgehog APRIL contains two cysteine residues (Cys(196) and Cys(211)), a furin protease cleavage site and a conserved putative N-glycosylation site (Asn(124)). Real-time quantitative PCR (qPCR) analysis revealed that heAPRIL could be detected in various tissues. MTT assays and flow cytometric analysis revealed that Nus-hesAPRIL and hesAPRIL could promote the survival/proliferation of splenic B cells. Laser scanning confocal microscopy analysis showed GFP-hesAPRIL could successfully bind to the APRIL receptors of lymphocytes.

摘要

在这里，我们描述了刺猬 Erinaceus europaeus 中 TNF 家族增殖诱导配体 (APRIL) 同源物 (命名为 heAPRIL) 的鉴定。刺猬 APRIL 包含两个半胱氨酸残基 (Cys(196) 和 Cys(211))、一个弗林蛋白酶切割位点和一个保守的潜在 N-糖基化位点 (Asn(124))。实时定量 PCR(qPCR)分析显示，heAPRIL 可在各种组织中检测到。MTT 分析和流式细胞术分析表明，Nus-hesAPRIL 和 hesAPRIL 可以促进脾 B 细胞的存活/增殖。激光扫描共聚焦显微镜分析表明，GFP-hesAPRIL 可以成功结合淋巴细胞的 APRIL 受体。

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1
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本文引用的文献

1
APRIL is critical for plasmablast survival in the bone marrow and poorly expressed by early-life bone marrow stromal cells.增殖诱导配体（APRIL）对骨髓中浆母细胞的存活至关重要，且在生命早期的骨髓基质细胞中表达水平较低。
Blood. 2008 Mar 1;111(5):2755-64. doi: 10.1182/blood-2007-09-110858. Epub 2008 Jan 7.
2
Two related ligands of the TNF family, BAFF and APRIL, in rabbit: molecular cloning, 3D modeling, and tissue distribution.兔体内肿瘤坏死因子家族的两个相关配体BAFF和APRIL：分子克隆、三维建模及组织分布
Cytokine. 2007 Sep;39(3):192-200. doi: 10.1016/j.cyto.2007.07.190. Epub 2007 Sep 5.
3
BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway.
B细胞活化因子（BAFF）和增殖诱导配体（APRIL）通过激活经典的核因子κB（NF-κB）信号通路来维持慢性淋巴细胞白血病B细胞的存活。
Blood. 2007 Jan 15;109(2):703-10. doi: 10.1182/blood-2006-06-027755. Epub 2006 Sep 14.
4
BAFF, APRIL and human B cell disorders.B细胞活化因子、增殖诱导配体与人类B细胞疾病
Semin Immunol. 2006 Oct;18(5):305-17. doi: 10.1016/j.smim.2006.04.004. Epub 2006 Aug 17.
5
APRIL and BAFF promote increased viability of replicating human B2 cells via mechanism involving cyclooxygenase 2.增殖诱导配体（APRIL）和B细胞活化因子（BAFF）通过涉及环氧化酶2的机制促进正在复制的人B2细胞的生存能力增加。
J Immunol. 2006 Jun 1;176(11):6736-51. doi: 10.4049/jimmunol.176.11.6736.
6
An APRIL to remember: novel TNF ligands as therapeutic targets.一个值得铭记的四月：新型肿瘤坏死因子配体作为治疗靶点。
Nat Rev Drug Discov. 2006 Mar;5(3):235-46. doi: 10.1038/nrd1982.
7
Selective activation of TACI by syndecan-2.Syndecan-2对TACI的选择性激活。
Blood. 2006 Apr 15;107(8):3235-42. doi: 10.1182/blood-2005-01-0256. Epub 2005 Dec 15.
8
Association between circulating levels of the novel TNF family members APRIL and BAFF and lymphoid organization in primary Sjögren's syndrome.新型肿瘤坏死因子家族成员增殖诱导配体（APRIL）和B淋巴细胞刺激因子（BAFF）的循环水平与原发性干燥综合征中淋巴组织的关联。
J Clin Immunol. 2005 May;25(3):189-201. doi: 10.1007/s10875-005-4091-5.
9
Nurselike cells express BAFF and APRIL, which can promote survival of chronic lymphocytic leukemia cells via a paracrine pathway distinct from that of SDF-1alpha.类滋养细胞表达BAFF和APRIL，它们可通过一种不同于SDF-1α的旁分泌途径促进慢性淋巴细胞白血病细胞的存活。
Blood. 2005 Aug 1;106(3):1012-20. doi: 10.1182/blood-2004-03-0889. Epub 2005 Apr 28.
10
Raised serum APRIL levels in patients with systemic lupus erythematosus.系统性红斑狼疮患者血清增殖诱导配体水平升高。
Ann Rheum Dis. 2005 Jul;64(7):1065-7. doi: 10.1136/ard.2004.022491. Epub 2004 Dec 2.