Norman Stephanie A, Goertz Caroline E C, Burek Kathy A, Quakenbush Lori T, Cornick Leslie A, Romano Tracy A, Spoon Tracey, Miller Woutrina, Beckett Laurel A, Hobbs Roderick C
Pathology, Microbiology, Immunology Department, School of Veterinary Medicine, University of California, Davis, One Shields Avenue, Davis, California 95616, USA.
J Wildl Dis. 2012 Jan;48(1):21-32. doi: 10.7589/0090-3558-48.1.21.
We collected blood from 18 beluga whales (Delphinapterus leucas), live-captured in Bristol Bay, Alaska, USA, in May and September 2008, to establish baseline hematologic and serum chemistry values and to determine whether there were significant differences in hematologic values by sex, season, size/age, or time during the capture period. Whole blood was collected within an average of 19 min (range=11-30 min) after the net was set for capture, and for eight animals, blood collection was repeated in a later season after between 80-100 min; all blood was processed within 12 hr. Mean hematocrit, chloride, creatinine, total protein, albumin, and alkaline phosphatase were significantly lower in May than they were in September, whereas mean corpuscular hemoglobin concentration, monocytes, phosphorous, magnesium, blood urea nitrogen, alanine aminotransferase, aspartate aminotransferase, γ-glutamyltranspeptidase, and creatinine kinase were significantly higher. Mean total protein, white blood cell count, neutrophils, and lymphocytes were significantly higher early in the capture period than they were later. No significant differences in blood analyte values were noted between males and females. Using overall body length as a proxy for age, larger (older) belugas had lower white blood cell, lymphocyte, and eosinophil counts as well as lower sodium, potassium, and calcium levels but higher creatinine levels than smaller belugas. These data provide values for hematology and serum chemistry for comparisons with other wild belugas.
2008年5月和9月，我们从美国阿拉斯加布里斯托尔湾活体捕获的18头白鲸（白鲸属）身上采集血液，以建立血液学和血清化学的基线值，并确定血液学值在性别、季节、体型/年龄或捕获期间的时间上是否存在显著差异。在设置捕获网后平均19分钟（范围=11 - 30分钟）内采集全血，对于8头动物，在80 - 100分钟后的晚些季节重复采集血液；所有血液均在12小时内进行处理。5月份的平均血细胞比容、氯离子、肌酐、总蛋白、白蛋白和碱性磷酸酶显著低于9月份，而平均红细胞血红蛋白浓度、单核细胞、磷、镁、血尿素氮、丙氨酸氨基转移酶、天冬氨酸氨基转移酶、γ-谷氨酰转肽酶和肌酸激酶则显著更高。捕获期早期的平均总蛋白、白细胞计数、中性粒细胞和淋巴细胞显著高于后期。未发现雄性和雌性之间血液分析物值存在显著差异。以总体长作为年龄的替代指标，体型较大（年龄较大）的白鲸白细胞、淋巴细胞和嗜酸性粒细胞计数较低，钠、钾和钙水平也较低，但肌酐水平高于体型较小的白鲸。这些数据为血液学和血清化学提供了数值，以便与其他野生白鲸进行比较。
