Forzán María J, Vanderstichel Raphaël V, Ogbuah Christopher T, Barta John R, Smith Todd G
Canadian Cooperative Wildlife Health Centre, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada.
J Wildl Dis. 2012 Jan;48(1):176-80. doi: 10.7589/0090-3558-48.1.176.
Collection of blood from amphibians, as in other classes of vertebrate animals, is essential to evaluate parameters of health, diagnose hemoparasitism, identify viral and bacterial pathogens, and measure antibodies. Various methods of blood collection have been described for amphibians. Most can be cumbersome (venipucture of femoral vein, ventral abdominal vein or lingual venus plexus) or result in pain or deleterious health consequences (cardiac puncture and toe-clipping). We describe an easy and practical technique to collect blood from frogs and toads that can be used in multiple species and is minimally invasive. The technique consists of puncturing either the facial or, less commonly, the musculo-cutaneous vein and collecting the blood with a capillary tube. These veins run dorsal and parallel to the maxillary bone and can be accessed by quick insertion and withdrawal of a needle through the skin between the upper jawline and the rostral or caudal side of the tympanum. The needle should be of 27 or 30 gauge for anurans weighing more or less than 25 g, respectively. Although the technique has been used by some amphibian researchers for years, it is little known by others and has never been fully described in a peer-reviewed publication.
与其他脊椎动物类群一样，采集两栖动物的血液对于评估健康参数、诊断血液寄生虫病、识别病毒和细菌病原体以及检测抗体至关重要。已经描述了多种用于两栖动物的采血方法。大多数方法可能很麻烦（股静脉、腹侧腹壁静脉或舌静脉丛的静脉穿刺），或者会导致疼痛或有害的健康后果（心脏穿刺和剪趾）。我们描述了一种简单实用的从青蛙和蟾蜍采集血液的技术，该技术可用于多个物种且微创性最小。该技术包括穿刺面部静脉，较少情况下穿刺肌皮静脉，并用毛细管采集血液。这些静脉位于上颌骨背侧并与之平行，可通过在上颌线与鼓膜的吻侧或尾侧之间快速将针头插入皮肤并拔出皮肤来进入。对于体重分别大于或小于25克的无尾目动物，针头应分别为27号或30号。尽管该技术已被一些两栖动物研究人员使用多年，但其他研究人员对此知之甚少，并且从未在同行评审的出版物中得到充分描述。
