Department of Medical Biochemistry and Cell Biology, University of Gothenburg , Box 440, 40530 Gothenburg, Sweden.
J Proteome Res. 2012 Mar 2;11(3):1879-90. doi: 10.1021/pr2010988. Epub 2012 Feb 2.
Goblet cells specialize in producing and secreting mucus with its main component, mucins. An inducible goblet-like cell line was used for the purification of the mucus vesicles stored in these cells by density gradient ultracentrifugation, and their proteome was analyzed by nanoLC-MS and MS/MS. Although the density of these vesicles coincides with others, it was possible to reveal a number of proteins that after immunolocalization on colon tissue and functional analyses were likely to be linked to the MUC2 vesicles. Most of the proteins were associated with the vesicle membrane or their outer surface. The ATP6AP2, previously suggested to be associated with vesicular proton pumps, was colocalized with MUC2 without other V-ATPase proteins and, thus, probably has roles in mucin vesicle function yet to be discovered. FAM62B, known to be a calcium-sensitive protein involved in vesicle fusion, also colocalized with the MUC2 vesicles and is probably involved in unknown ways in the later events of the MUC2 vesicles and their secretion.
杯状细胞专门负责产生和分泌黏液，其主要成分是黏蛋白。通过密度梯度超速离心，从可诱导的杯状细胞系中纯化储存在这些细胞中的黏液小泡，并通过纳升液相色谱-质谱联用（nanoLC-MS）和串联质谱（MS/MS）分析其蛋白质组。尽管这些小泡的密度与其他小泡相同，但仍有可能发现一些蛋白质，这些蛋白质在免疫定位到结肠组织上并进行功能分析后，很可能与 MUC2 小泡有关。大多数蛋白质与小泡膜或其外表面相关。ATP6AP2 先前被认为与囊泡质子泵有关，它与 MUC2 共定位，而没有其他 V-ATPase 蛋白，因此可能在尚未发现的粘蛋白囊泡功能中发挥作用。FAM62B 是一种已知的钙敏感性蛋白，参与囊泡融合，也与 MUC2 囊泡共定位，可能以未知的方式参与 MUC2 囊泡及其分泌的后期事件。
