Department of Obstetrics and Gynecology, Shanghai Jiaotong University No. 6 People's Hospital, Shanghai, PR China.
Gynecol Obstet Invest. 2012;73(3):201-10. doi: 10.1159/000332401. Epub 2012 Jan 13.
BACKGROUND/AIMS: To investigate cytotrophoblast (CTB) invasive ability and human uterine spiral artery smooth muscle cell (HUSASMC) apoptosis in a coculture model with serum from preeclamptic pregnancies.
Transwell migration assay was used to detect the invasive ability of CTBs. Cocultured CTBs and HUSASMCs were incubated with normal or preeclamptic serum for 24 h. Monocultures of CTBs and HUSASMCs were treated identically to the cocultures and served as controls. HUSASMC viability and apoptosis rates were determined by MTT and annexin V-FITC assays. The expressions of Fas ligand (FasL) mRNA in CTBs and Fas mRNA in HUSASMCs were detected by RT-PCR. The expression of the Fas protein in HUSASMCs was detected by Western blotting.
In a model of CTBs cocultured with HUSASMCs, preeclamptic serum effectively decreased the invasive ability and FasL mRNA expression of the CTBs. Preeclampsia serum also increased HUSASMC viability, decreased their apoptotic rate, and decreased the expression of Fas mRNA and protein.
The abnormal invasive ability of CTBs and decreased expression of the Fas/FasL system may be directly involved in the defective remodeling of the uterine spiral arteries during preeclampsia. Furthermore, the decrease in HUSASMC apoptosis may be related to the abnormal expression of Fas/FasL.
背景/目的：研究子痫前期患者血清对滋养细胞侵袭能力和人子宫螺旋动脉平滑肌细胞（HUSASMC）凋亡的影响。
采用 Transwell 迁移实验检测滋养细胞的侵袭能力。将 CTB 和 HUSASMC 共培养，用正常或子痫前期血清孵育 24 小时。对 CTB 和 HUSASMC 的单纯培养也进行相同的处理，作为对照组。通过 MTT 和 annexin V-FITC 检测 HUSASMC 活力和凋亡率。用 RT-PCR 检测 CTB 中 Fas 配体（FasL）mRNA 和 HUSASMC 中 Fas mRNA 的表达。用 Western blot 检测 HUSASMC 中 Fas 蛋白的表达。
在 CTB 与 HUSASMC 共培养模型中，子痫前期血清可有效降低 CTB 的侵袭能力和 FasL mRNA 的表达。子痫前期血清还增加了 HUSASMC 的活力，降低了其凋亡率，下调了 Fas mRNA 和蛋白的表达。
滋养细胞异常的侵袭能力和 Fas/FasL 系统表达降低可能直接参与子痫前期子宫螺旋动脉的不良重塑。此外，HUSASMC 凋亡减少可能与 Fas/FasL 的异常表达有关。
