Borges Marcus Nascimento, Facina Gil, Silva Ismael Dale Cotrin Guerreiro, Waitzberg Angela Flávia Logullo, Nazario Afonso Celso Pinto
Department of Gynecology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
Sao Paulo Med J. 2011 Dec;129(6):402-9. doi: 10.1590/s1516-31802011000600006.
Dendritic cell maturation is considered essential for starting an immune response. The CD83 antigen is an important marker of dendritic cell maturation. The objectives here were to analyze CD83 antigen expression in human breast fibroadenoma and breast tissue adjacent to the lesion and to identify clinical factors that might influence this expression.
This was a retrospective study at a public university hospital, in which 29 histopathological samples of breast fibroadenoma and adjacent breast tissue, from 28 women of reproductive age, were analyzed.
The immunohistochemistry method was used to analyze the cell expression of the antigen. The antigen expression in the cells was evaluated by means of random manual counting using an optical microscope.
Positive expression of the CD83 antigen in the epithelial cells of the fibroadenoma (365.52; standard deviation ± 133.13) in relation to the adjacent breast tissue cells (189.59; standard deviation ± 140.75) was statistically larger (P < 0.001). Several clinical features were analyzed, but only parity was shown to influence CD83 antigen expression in the adjacent breast tissue, such that positive expression was more evident in nulliparous women (P = 0.042).
The expression of the CD83 antigen in the fibroadenoma was positive and greater than in the adjacent breast tissue. Positive expression of the antigen in the adjacent breast tissue was influenced by parity, and was significantly more evident in nulliparous women.
树突状细胞成熟被认为是启动免疫反应的关键。CD83抗原是树突状细胞成熟的重要标志物。本研究旨在分析CD83抗原在人乳腺纤维腺瘤及病变周围乳腺组织中的表达情况，并确定可能影响该表达的临床因素。
这是一项在公立大学医院开展的回顾性研究，分析了28名育龄女性的29份乳腺纤维腺瘤及相邻乳腺组织的组织病理学样本。
采用免疫组织化学方法分析抗原的细胞表达情况。通过光学显微镜随机手动计数来评估细胞中的抗原表达。
与相邻乳腺组织细胞（189.59；标准差±140.75）相比，纤维腺瘤上皮细胞中CD83抗原的阳性表达（365.52；标准差±133.13）在统计学上更高（P < 0.001）。分析了多个临床特征，但只有产次被证明会影响相邻乳腺组织中CD83抗原的表达，未生育女性的阳性表达更明显（P = 0.042）。
纤维腺瘤中CD83抗原的表达呈阳性，且高于相邻乳腺组织。相邻乳腺组织中抗原的阳性表达受产次影响，在未生育女性中明显更显著。
