Benov L C, Benchev I C, Monovich O H
Department of Biophysics, Medical Institute, Stara Zagora, Bulgaria.
Chem Biol Interact. 1990;76(3):321-32. doi: 10.1016/0009-2797(90)90099-9.
The effect of thiol antidotes 2,3-dimercapto-1-propanesulfonic acid (DMPS) and D-penicillamine (PA) on lipid peroxidation and on activities of some protecting enzymes in blood, liver and kidneys of mercury-poisoned rats has been studied. It has been found that Hg-poisoning is associated with increased lipid peroxidation in the liver and in the kidneys and with inactivation of superoxide dismutase (SOD) and catalase. Inhibition of SOD is caused by thiols treatment too, but in this case acceleration of lipid peroxidation has not been observed. Evidence is presented that in the liver, protection against mercury-induced lipid peroxidation is afforded by both thiols, while in the kidneys only PA has protective effect. In in vitro experiments it has been demonstrated that both antidotes can act as O2- scavengers and lipid peroxidation inhibitors, but PA is significantly more effective. On the basis of the obtained results a conclusion is drawn that in addition to the metal-removing ability, the antioxidant properties of the chelating agents may play an important role in manifestation of their beneficial effect in metal intoxications.
研究了硫醇解毒剂2,3-二巯基-1-丙磺酸钠（DMPS）和D-青霉胺（PA）对汞中毒大鼠血液、肝脏和肾脏中脂质过氧化以及某些保护酶活性的影响。研究发现，汞中毒与肝脏和肾脏中脂质过氧化增加以及超氧化物歧化酶（SOD）和过氧化氢酶失活有关。硫醇处理也会导致SOD受到抑制，但在这种情况下未观察到脂质过氧化加速。有证据表明，在肝脏中，两种硫醇都能保护机体免受汞诱导的脂质过氧化，而在肾脏中只有PA具有保护作用。体外实验表明，两种解毒剂都可作为超氧阴离子清除剂和脂质过氧化抑制剂，但PA的效果明显更好。根据所得结果得出结论，除了金属清除能力外，螯合剂的抗氧化特性在其对金属中毒有益作用的表现中可能起重要作用。
