Neant Isabelle, Deisig Nina, Scerbo Pierluigi, Leclerc Catherine, Moreau Marc
Centre de Biologie du Développement, CNRS, UMR5547 and GDR2688, Université P. Sabatier, Toulouse, France.
Int J Dev Biol. 2011;55(10-12):923-31. doi: 10.1387/ijdb.103253in.
In amphibian embryos, calcium (Ca(2+)) signalling is a necessary and sufficient event to induce neural fate. Transient elevations of [Ca(2+)]i are recorded in neural tissue precursor cells in whole embryos during gastrulation. Using a subtractive cDNA library between control ectoderm (animal caps) and ectoderm induced toward a neural fate by Ca(2+) release, we have isolated several Ca(2+)-induced target genes. Among the isolated genes, Xp54nrb encodes a protein which exhibits the RRM domains characteristic of RNA binding proteins, and is implicated in pre-mRNA splicing steps. Here we show that the Xp54nrb transcripts are expressed throughout early developmental stages, specifically in the neural and sensorial territories and that Xp54nrb could be involved in anterior neural patterning.
在两栖动物胚胎中，钙（Ca(2+)）信号传导是诱导神经命运的必要且充分条件。在原肠胚形成过程中，整个胚胎的神经组织前体细胞中记录到[Ca(2+)]i的短暂升高。利用对照外胚层（动物帽）和通过Ca(2+)释放诱导为神经命运的外胚层之间的消减cDNA文库，我们分离出了几个Ca(2+)诱导的靶基因。在分离出的基因中，Xp54nrb编码一种蛋白质，该蛋白质具有RNA结合蛋白特有的RRM结构域，并参与前体mRNA剪接步骤。在这里我们表明，Xp54nrb转录本在整个早期发育阶段都有表达，特别是在神经和感觉区域，并且Xp54nrb可能参与前脑的神经模式形成。
