Max Planck Institute for Intelligent Systems, Department of New Materials and Biosystems, Stuttgart, Germany.
Lab Chip. 2012 Mar 7;12(5):916-22. doi: 10.1039/c2lc20971c. Epub 2012 Jan 17.
Despite its tremendous high-throughput screening capabilities, widespread applications of droplet-based microfluidics are still limited by the poor availability of appropriate analytical assays. Here we report on a novel sensor method that exploits the osmosis-driven change in droplet size as a quantitative and label-free marker for reactions inside the droplets. We present an analysis of the underlying mechanism and apply the method for monitoring metabolic activity at a single-cell level.
尽管基于液滴的微流控技术具有巨大的高通量筛选能力，但由于缺乏合适的分析检测方法，其广泛应用仍然受到限制。在这里，我们报告了一种新的传感器方法，该方法利用渗透压驱动的液滴尺寸变化作为液滴内部反应的定量和无标记标记物。我们提出了对潜在机制的分析，并将该方法应用于单细胞水平的代谢活性监测。
