粪便中人类血液的免疫化学检测。
Immunochemical detection of human blood in feces.
作者信息
Uchida K, Matsuse R, Miyachi N, Okuda S, Tomita S, Miyoshi H, Hirata I, Tsumoto S, Ohshiba S
机构信息
Kyoto Medical Science Laboratory Incorporation, Japan.
出版信息
Clin Chim Acta. 1990 Aug 31;189(3):267-74. doi: 10.1016/0009-8981(90)90308-f.
We have developed a new immunochemical test for fecal occult blood utilizing enzyme-linked immunosorbent assay (ELISA) of human hemoglobin (HbAo) and transferrin (Tf) simultaneously. The ELISA had a sensitivity of about 15 ng/ml Hb, and the measurable range was 1.5-750 micrograms Hb per g feces. The stability of Tf in feces was greater than that of Hb. In 17 out of 18 patients with colon cancer, 8 out of 15 patients with colon polyps, and 11 out of 20 patients with upper-gastrointestinal disorders. The Hb and Tf values were more than 10 micrograms/g feces, in terms of Hb concentration. The ELISA for human fecal HbAo and Tf might be useful for the diagnosis of gastrointestinal disorders.
我们开发了一种新的粪便潜血免疫化学检测方法，该方法同时利用酶联免疫吸附测定（ELISA）对人血红蛋白（HbAo）和转铁蛋白（Tf）进行检测。该ELISA对血红蛋白的灵敏度约为15 ng/ml，可测量范围为每克粪便中含1.5 - 750微克血红蛋白。转铁蛋白在粪便中的稳定性高于血红蛋白。在18例结肠癌患者中有17例、15例结肠息肉患者中有8例以及20例上消化道疾病患者中有11例，就血红蛋白浓度而言，其血红蛋白和转铁蛋白值均超过每克粪便10微克。检测人粪便中HbAo和Tf的ELISA可能对胃肠道疾病的诊断有用。
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