Peñalva A, Gaztambide S, Vazquez J A, Dieguez C, Casanueva F F
Department of Medicine, Hospital General de Galicia, Spain.
Clin Endocrinol (Oxf). 1990 Aug;33(2):171-6. doi: 10.1111/j.1365-2265.1990.tb00480.x.
In order to explore the mechanisms by which free fatty acids (FFA) inhibit GH secretion, we studied the effect of the acetylcholinesterase inhibitor pyridostigmine (120 mg p.o.) on the FFA blockade of GH responses to the administration of GHRH (100 micrograms i.v.) in seven normal subjects. GHRH-induced GH secretion was significantly reduced following elevation of circulating FFA levels by lipid-heparin infusion and significantly potentiated by previous pyridostigmine treatment. Peak GH levels following combined administration of pyridostigmine plus lipid-heparin plus GHRH were significantly higher (P less than 0.01) than after GHRH alone and significantly lower than after pyridostigmine plus GHRH (P less than 0.01). In conclusion, central cholinergic activation by pyridostigmine, with the presumed reduction in somatostatin discharge, reversed the blocking effect of FFA on GHRH-stimulated GH release. Conversely, FFA were able to reduce even a maximal GH stimulation by pyridostigmine plus GHRH.
为了探究游离脂肪酸（FFA）抑制生长激素（GH）分泌的机制，我们研究了乙酰胆碱酯酶抑制剂吡啶斯的明（口服120毫克）对7名正常受试者中FFA阻断GH对生长激素释放激素（GHRH，静脉注射100微克）反应的影响。通过脂质 - 肝素输注使循环FFA水平升高后，GHRH诱导的GH分泌显著降低，而预先给予吡啶斯的明治疗则使其显著增强。吡啶斯的明加脂质 - 肝素加GHRH联合给药后的GH峰值水平显著高于单独给予GHRH后（P < 0.01），且显著低于吡啶斯的明加GHRH后（P < 0.01）。总之，吡啶斯的明引起的中枢胆碱能激活，推测会减少生长抑素的释放，逆转了FFA对GHRH刺激的GH释放的阻断作用。相反，FFA甚至能够降低吡啶斯的明加GHRH对GH的最大刺激作用。
