Depth-resolved mapping of tissue mechanical properties using a novel optical approach.

Progression of most diseases, such as atherosclerosis, cancer, neurodegenerative disease and osteoarthritis is accompanied with drastic changes in biomechanics of tissue. Hence, non-contact and non-invasive technologies for 3-dimensional mapping of tissue biomechanics are invaluable for diagnostic purposes. Laser speckle Microrheology (LSM) is developed in our lab to enable high resolution mechanical evaluation of tissue. To this end, the tissue sample is illuminated by a coherent and focused laser beam and the back-scattered laser speckle pattern is spatio-temporally processed to extract a color-map of τ, which is the decay time constant of intensity decorrelation at each pixel in the image plane. Time constant, τ, is proven to be closely correlated with tissue mechanical properties. In this paper we validate the theoretical basis for LSM technology and investigate the potential for acquiring depth-resolved information from a light-scattering point of view. The patch analysis approach is introduced and the inter-relation between τ, number of scattering events, and penetration depth is explored for each patch. Axial variation of τ is characterized for two sample arterial regions and in-depth changes of mechanical properties are characterized. Finally, the required corrective measures are discussed.