Machalińska Anna, Safranow Krzysztof, Sylwestrzak Zofia, Szmatłoch Krzysztof, Kuprjanowicz Leszek, Karczewicz Danuta
Z Katedry i Zakładu Histologii i Embriologii Pomorskiego Uniwersytetu Medycznego w Szczecinie.
Klin Oczna. 2011;113(7-9):228-32.
In recent years, emerging data support the concept of endothelial dysfunction in the course of age-related macular degeneration (AMD). Circulating endothelial cells (CECs) are desquamated mature cells that have detached from the intimal monolayer in response to endothelial injury. In this study we sought to explore the potential role of endothelial dysfunction in pathogenesis of AMD by measuring the concentration of CECs in peripheral blood of AMD patients.
Peripheral blood samples from 31 patients with diagnosed dry AMD and 46 patients with neovascular AMD were collected. Forty six, age- and sex-matched volunteers without AMD were enrolled as a control group. CECs were counted and analyzed by flow cytometry.
Multivariate analyses of patients and controls adjusted for age, gender, presence of ischemic heart disease, hypertension and smoking (current or past) revealed that both wet (beta = +0.45, p = 0.0003) and dry (beta = +0.28, p = 0.027) forms of AMD are independent factors associated with higher number of CECs.
Our results suggest that there is an endothelial alteration accompanying AMD. Increased numbers of CECs AMD patients reflect a severe vascular disturbance and may contribute to the disease process. These findings can help expand our knowledge of the pathogenic mechanisms of AMD and may be relevant to the potential treatment of this disease.
近年来，新出现的数据支持了年龄相关性黄斑变性（AMD）病程中存在内皮功能障碍的概念。循环内皮细胞（CEC）是因内皮损伤而从内膜单层脱落的成熟脱屑细胞。在本研究中，我们试图通过测量AMD患者外周血中CEC的浓度，探讨内皮功能障碍在AMD发病机制中的潜在作用。
收集31例确诊为干性AMD患者和46例新生血管性AMD患者的外周血样本。招募46名年龄和性别匹配、无AMD的志愿者作为对照组。通过流式细胞术对CEC进行计数和分析。
对患者和对照组进行多因素分析，校正年龄、性别、缺血性心脏病、高血压和吸烟（当前或既往）后发现，湿性（β = +0.45，p = 0.0003）和干性（β = +0.28，p = 0.027）AMD均是与CEC数量增加相关的独立因素。
我们的结果表明，AMD伴有内皮改变。AMD患者CEC数量增加反映了严重的血管紊乱，可能促成疾病进程。这些发现有助于扩展我们对AMD致病机制的认识，可能与该疾病的潜在治疗相关。
