Zheng Jinwei, Zheng Changjian, Cao Hong, Li Jun, Lian Qingquan
Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325027, China.
Zhongguo Zhong Yao Za Zhi. 2011 Sep;36(18):2552-6.
To investigate the effects of curcumin on the behavior of chronic constrictive injury (CCI) rats and the CX3CR1 expression in spinal cord dorsal horn and dorsal root ganglia (DRG).
Seventy-two male SD rats were randomly divided into 4 groups: 1) Sham operation group (Sham); 2) Chronic constrictive injury group (CCI); 3) Curcumin treated group (Cur), administrated with curcumin 100 mg x kg(-1) x d(-1) ip for 14 days after CCI; 4) Solvent contrast group (SC), administrated with an equal volume of solvent for 14 days after CCI. Paw thermal withdrawal (PTWL) and paw mechanical withdrawal threshold (PMWT) were measured on 2 pre-operative and 1, 3, 5, 7, 10, 14 post-operative days respectively. The lumbar segments L4-5 of the spinal cord and the L4, L5 DRG were removed at 3, 7, 14 days after surgery. The expression of CX3CR1 was determined by immunohistochemical staining.
Compared with Sham group, PTWL and PMWT in CCI group were significantly lower on each post-operative day (P<0.01), which reached a nadir on the 3rd day after CCI (PTWL was 6.5 +/- 1.1, PMWT was 22.6 +/- 5.1), and the expression of CX3CR1 were markedly increased in spinal cord dorsal horn and DRG. In Cur group, PTWL were higher than in CCI group on 7, 10, 14 post-operative day (P<0.05), and PMWT were higher than those in CCI group on 10 and 14 post-operative day (P<0.05). The administration of curcumin could significantly attenuate the activation of CX3CR1 induced by CCI.
The study suggests that curcumin ameliorates the CCI-induced neuropathic pain, probably by attenuating the expression of CX3CR1 in spinal cord dorsal horn and dorsal root ganglia.
探讨姜黄素对慢性压迫性损伤（CCI）大鼠行为及脊髓背角和背根神经节（DRG）中CX3CR1表达的影响。
将72只雄性SD大鼠随机分为4组：1）假手术组（Sham）；2）慢性压迫性损伤组（CCI）；3）姜黄素治疗组（Cur），在CCI后腹腔注射100 mg·kg⁻¹·d⁻¹姜黄素，共14天；4）溶剂对照组（SC），在CCI后给予等体积溶剂，共14天。分别在术前2天及术后1、3、5、7、10、14天测量爪部热缩足潜伏期（PTWL）和爪部机械缩足阈值（PMWT）。术后3、7、14天取脊髓腰段L4-5及L4、L5 DRG，采用免疫组织化学染色法检测CX3CR1的表达。
与Sham组相比，CCI组术后各天的PTWL和PMWT均显著降低（P<0.01），在CCI后第3天达到最低点（PTWL为6.5±1.1，PMWT为22.6±5.1），脊髓背角和DRG中CX3CR1的表达明显增加。在Cur组，术后7、10、14天的PTWL高于CCI组（P<0.05），术后10和14天的PMWT高于CCI组（P<0.05）。姜黄素给药可显著减轻CCI诱导的CX3CR1激活。
该研究表明姜黄素可改善CCI诱导的神经性疼痛，可能是通过减弱脊髓背角和背根神经节中CX3CR1的表达来实现的。
