通过氮杂电环化反应进行细胞表面生物素化：一种易于操作且用途广泛的活细胞标记方法。
Cell surface biotinylation by azaelectrocyclization: easy-handling and versatile approach for living cell labeling.
机构信息
Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka-shi, Osaka 560-0043, Japan.
出版信息
Bioorg Med Chem. 2012 Mar 15;20(6):1865-8. doi: 10.1016/j.bmc.2011.12.043. Epub 2011 Dec 30.
PMID:22257530
Abstract
Versatile method for living cell labeling has been established. Cell surfaces are initially biotinylated by azaelectrocyclization, and then treated with the fluorescence-labeled avidin or the anti-biotin antibody.
摘要
建立了一种用于活细胞标记的多功能方法。细胞表面最初通过氮杂电环化反应进行生物素化，然后用荧光标记的亲和素或抗生物素抗体处理。
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