Ribeiro J M, Sillero A
Departamento de Bioquímica, Facultad de Medicina, Universidad Autónoma, Madrid.
Comput Biol Med. 1990;20(4):235-42. doi: 10.1016/0010-4825(90)90048-t.
The isoelectric point (pI) of a macromolecule containing any number of acid-base residues can be expressed as a polynomial whose coefficients are related to both the number of acid-base residues present in the molecule and their K values. Polynomials of degree higher than 5 are too complicated to be solved manually. An algorithm is here presented which allows, with few sentences written in BASIC, the calculation of the polynomial coefficients. With this knowledge and using standard polynomial solving programs, the isoelectric point of the macromolecule can be calculated.
