Reproductive Medical Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Reprod Sci. 2012 Feb;19(2):216-20. doi: 10.1177/1933719111424450. Epub 2012 Jan 17.
The purpose of this study was to explore the effects of cumulus cells on vitreous cryopreservation of human mature oocytes and clinical pregnancy outcomes. The study was divided into group A (cumulus cells were removed from the oocytes before freezing) containing 24 participants and 193 oocytes and group B (cumulus cells were retained with the oocytes before freezing) containing 26 participants and 240 oocytes. Based on no significant differences in age, duration of infertility, infertile causes, and number of retrieved oocytes between both groups when oocytes were retrieved from infertile women, we found that the survival rate of post thaw oocytes (88% vs 58%), cleavage rate (80% vs 56%), and high-quality embryo rate (75% vs 59%) were significantly higher in group B than in group A. Under the conditions that there were no significant differences between the 2 groups in the general status of the participants undergoing embryo transfer, the embryo implantation rate (37% vs 15%) and the clinical pregnancy rate (50% vs 17%) were significantly higher in group B than in group A, all with Ps < .05. We conclude that the retention of cumulus cells can improve the developmental competence of vitrified-thawed human mature oocytes and clinical pregnancy outcomes.
本研究旨在探讨卵丘细胞对人成熟卵母细胞玻璃化冷冻保存及临床妊娠结局的影响。研究分为 A 组（卵母细胞冷冻前去除卵丘细胞），包含 24 名参与者和 193 枚卵母细胞；B 组（卵母细胞冷冻前保留卵丘细胞），包含 26 名参与者和 240 枚卵母细胞。根据两组在接受不孕女性取卵时的年龄、不孕持续时间、不孕原因和获卵数无显著差异，我们发现 B 组的卵母细胞解冻后存活率（88%比 58%）、卵裂率（80%比 56%）和优质胚胎率（75%比 59%）均显著高于 A 组。在胚胎移植的参与者一般情况两组间无显著差异的情况下，B 组的胚胎着床率（37%比 15%）和临床妊娠率（50%比 17%）均显著高于 A 组，均 P <.05。我们得出结论，保留卵丘细胞可以提高玻璃化冷冻解冻后人类成熟卵母细胞的发育能力和临床妊娠结局。
