Department of Water Supply Engineering, National Institute of Public Health, 2-3-6 Minami, Wako, Saitama 351-0197, Japan.
Water Sci Technol. 2012;65(3):581-8. doi: 10.2166/wst.2012.888.
In this study, the effectiveness of aerobic granular sludge as seed sludge for rapid start-up of nitrifying processes was investigated using a laboratory-scale continuous stirred-tank reactor (CSTR) fed with completely inorganic wastewater which contained a high concentration of ammonia. Even when a large amount of granular biomass was inoculated in the reactor, and the characteristics of influent wastewater were abruptly changed, excess biomass washout was not observed, and biomass concentration was kept high at the start-up period due to high settling ability of the aerobic granular sludge. As a result, an ammonia removal rate immediately increased and reached more than 1.0 kg N/m(3)/d within 20 days and up to 1.8 kg N/m(3)/d on day 39. Subsequently, high rate nitritation was stably attained during 100 days. However, nitrite accumulation had been observed for 140 days before attaining complete nitrification to nitrate. Fluorescence in situ hybridization analysis revealed the increase in amount of ammonia-oxidizing bacteria which existed in the outer edge of the granular sludge during the start-up period. This microbial ecological change would make it possible to attain high rate ammonia removal.
在这项研究中，使用实验室规模的连续搅拌槽式反应器（CSTR），以含有高浓度氨的完全无机废水作为接种污泥，考察了好氧颗粒污泥作为快速启动硝化过程的接种污泥的效果。即使在反应器中接种了大量的颗粒生物量，并且进水废水的特性突然发生变化，也没有观察到过量的生物量洗出，并且由于好氧颗粒污泥具有较高的沉降能力，在启动期间保持了较高的生物量浓度。结果，氨去除率立即增加，在 20 天内达到 1.0 公斤 N/m³/天以上，在第 39 天达到 1.8 公斤 N/m³/天。随后，在达到完全硝化到硝酸盐之前，100 天内稳定实现了高硝化速率。然而，在达到完全硝化之前，亚硝酸盐积累已经观察到 140 天。荧光原位杂交分析表明，在启动期间，氨氧化菌的数量在颗粒污泥的外边缘增加。这种微生物生态变化使得实现高氨去除率成为可能。
