Department of Nutritional Physiology and Feeding, Faculty of Animal Science and Aquaculture, Agricultural University of Athens, 118 55, Athens, Greece.
Endocrine. 2012 Jun;41(3):501-11. doi: 10.1007/s12020-012-9594-2. Epub 2012 Jan 19.
Thyroid hormones increase energy expenditure and bone turnover in vivo. To study whether 3,3',5-triiodo-l-thyronine (T₃) stimulates the uptake of glucose in osteoblastic cells, PyMS (a cell line derived from rat bone) cells were kept in serum-free culture medium and treated with T₃. We measured [1-¹⁴C]-2-deoxy-D: -glucose (2DG) uptake and looked for expression of the high-affinity glucose transporters GLUT1 and GLUT3 by northern and western analysis. T₃ did not influence the cell number but slightly (1.3-fold) increased the protein content of the cell cultures. 2DG uptake was low in serum-deprived cell cultures and was increased by T₃ (up to 2.5-fold at 1 nmol l⁻¹ after 4 days) in a dose- and time-dependent manner. Triiodothyronine at 1 nmol l⁻¹ increased GLUT1 and GLUT3 abundance in membranes. Therefore, increased glucose uptake induced by T₃ in osteoblasts may be mediated by the known high-affinity glucose transporters GLUT1 and GLUT3.
甲状腺激素可增加体内能量消耗和骨转换。为了研究 3,3',5-三碘甲腺原氨酸（T₃）是否刺激成骨细胞摄取葡萄糖，将 PyMS（一种源自大鼠骨的细胞系）细胞在无血清培养物中培养，并添加 T₃进行处理。我们测量了[1-¹⁴C]-2-脱氧-D：-葡萄糖（2DG）的摄取，并通过 Northern 和 Western 分析寻找高亲和力葡萄糖转运蛋白 GLUT1 和 GLUT3 的表达。T₃ 不影响细胞数量，但略微（1.3 倍）增加了细胞培养物的蛋白质含量。在血清剥夺的细胞培养物中，2DG 的摄取量较低，T₃ 以剂量和时间依赖的方式增加摄取（4 天后 1 nmol l⁻¹ 时增加 2.5 倍）。1 nmol l⁻¹ 的三碘甲状腺原氨酸增加了膜中的 GLUT1 和 GLUT3 丰度。因此，T₃ 在成骨细胞中诱导的葡萄糖摄取增加可能是由已知的高亲和力葡萄糖转运蛋白 GLUT1 和 GLUT3 介导的。
