[北五味子性别连锁分子标记的建立]
[Establishment of molecular markers linked to sex in Schisandra sphenanthera].
作者信息
Xiong Yu-Ting, Wang Xin-Sheng, Chen Ya-Xiang, Zong Wen-Hui, Yang Xiao, Gu Wei
机构信息
Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Xi'an 710062, China.
出版信息
Zhong Yao Cai. 2011 Sep;34(9):1339-42.
OBJECTIVE
To establish the gender-related RAPD markers in Schisandra sphenanthera.
METHODS
The genomic DNA was extracted from the young leaves of male and female Schisandra sphenanthera by modified CTAB method. The gender differences in the genome were studied by RAPD which was optimized by the single factor and orthogonal experiments.
RESULTS
25 microL total volume included Mg2+ of 2.5 mmol/L, dNTPs of 0.08 mmol/L, primer of 0.6 micromol/L, Taq enzyme 1.5 U, DNA template 60 ng, annealing temperature 41.3 degrees C, 35 cycles. In 400 random primers, only a male specific band 541 bp was generated by S353.
CONCLUSION
The marker can be used as the basis of gender identification.
目的
建立南五味子性别相关的随机扩增多态性DNA（RAPD）标记。
方法
采用改良CTAB法从南五味子雌雄株幼叶中提取基因组DNA。通过单因素和正交试验优化RAPD，研究基因组中的性别差异。
结果
25 μL总体积中含2.5 mmol/L Mg2+、0.08 mmol/L dNTPs、0.6 μmol/L引物、1.5 U Taq酶、60 ng DNA模板，退火温度41.3℃，35个循环。在400条随机引物中，S353仅产生一条541 bp的雄性特异性条带。
结论
该标记可作为性别鉴定的依据。
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