对西班牙绵羊中十二指肠贾第虫的多位点基因分型显示出高度的异质性。
Multilocus genotyping of Giardia duodenalis in lambs from Spain reveals a high heterogeneity.
机构信息
Departamento de Producción Animal, Sanidad Animal y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad CEU Cardenal-Herrera, Avenida Seminario S/N, 46113 Moncada, Valencia, Spain.
出版信息
Res Vet Sci. 2012 Oct;93(2):836-42. doi: 10.1016/j.rvsc.2011.12.012. Epub 2012 Jan 18.
Fecal specimens from 120 lambs in Valencia (Spain) were analyzed for Giardia duodenalis by IFA and nested-PCR using the beta giardin (bg), glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi) and small subunit ribosomal RNA (ssurRNA) genes. The highest prevalence was obtained using the ssurRNA gene (89.2%), whereas values from other techniques ranged from 64.1% to 69.2%. Sequences of the ssurRNA showed a high proportion of assemblage A or mixed assemblage A/E samples (55.1% and 25.2%, respectively). When the other 3 loci were analyzed, between 6.5% and 15.4% were found to be assemblage A or A/E, respectively. Nested PCR for the tpi gene was the most variable of the targets employed. Twelve new sequences of gdh and tpi for G. duodenalis from sheep were found. Multilocus genotyping resulted in 63 patterns from the 71 samples sequenced at the four loci. This high variability among isolates possibly reflects the high frequency of mixed infections.
对西班牙瓦伦西亚的 120 只羔羊的粪便样本进行了贾第虫属十二指肠的间接免疫荧光法和巢式 PCR 分析，使用β-贝氏隐孢子虫（bg）、谷氨酸脱氢酶（gdh）、磷酸丙糖异构酶（tpi）和小亚基核糖体 RNA（ssurRNA）基因。使用 ssurRNA 基因获得的阳性率最高（89.2%），而其他技术的阳性率范围为 64.1%至 69.2%。ssurRNA 序列显示出高比例的 A 或混合 A/E 组样本（分别为 55.1%和 25.2%）。当分析其他 3 个基因座时，发现分别有 6.5%和 15.4%为 A 或 A/E 组。tpi 基因的巢式 PCR 是最具变异性的靶标。从绵羊中发现了 12 个新的 gdh 和 tpi 基因序列。对这四个基因座进行测序的 71 个样本进行了多位点基因分型，得到了 63 种模式。这种高变异性可能反映了混合感染的高频率。
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