Aebersold R, Leavitt J
Biomedical Research Centre, University of British Columbia, Vancouver, Canada.
Electrophoresis. 1990 Jul;11(7):517-27. doi: 10.1002/elps.1150110702.
Improved technologies or the synergistic use of complementary methods enhance the efficiency of research and permit the exploration of new approaches for the investigation of complex problems. High sensitivity protein sequence analysis and polyacrylamide gel electrophoresis are such complementary methods. Here we summarize the current status of high sensitivity sequence analysis of proteins separated in polyacrylamide gels and discuss strategies by which this technology can enhance biological research by generating new approaches for the solution of complex, multifacetted problems. Finally, we outline imminent technological advances in the area of high sensitivity protein sequence analysis and argue that further technological developments will ultimately lead to the generation of an integrated protein database (containing structural and functional as well as physiological information in an easily accessible form) of all the proteins separated by high resolution two-dimensional gel electrophoresis.
改进的技术或互补方法的协同使用提高了研究效率，并允许探索研究复杂问题的新方法。高灵敏度蛋白质序列分析和聚丙烯酰胺凝胶电泳就是这样的互补方法。在这里，我们总结了在聚丙烯酰胺凝胶中分离的蛋白质的高灵敏度序列分析的现状，并讨论了该技术通过产生解决复杂多方面问题的新方法来加强生物学研究的策略。最后，我们概述了高灵敏度蛋白质序列分析领域即将取得的技术进展，并认为进一步的技术发展最终将导致生成一个整合的蛋白质数据库（以易于获取的形式包含结构、功能以及生理信息），该数据库涵盖通过高分辨率二维凝胶电泳分离的所有蛋白质。
