Center for Biofluid and Biomimic Research, Pohang University of Science and Technology, Pohang, South Korea.
Ultrasound Med Biol. 2012 Mar;38(3):468-75. doi: 10.1016/j.ultrasmedbio.2011.11.013. Epub 2012 Jan 21.
This study aims to investigate the feasibility of using high-frequency ultrasound (HFUS) for simultaneous monitoring of blood coagulation and red blood cell (RBC) aggregation. Using a 35-MHz ultrasound scanner, ultrasound speckle data were acquired from whole blood samples of three experimental groups of rats, including 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS)-treated, noncoagulation and normal control groups. The variations of blood echogenicity, the shape parameters of probability distribution of speckle intensity (skewness and kurtosis) and the correlation coefficient between two consecutive speckle data were calculated as a function of time starting from immediately after taking blood. The blood echogenicity increases rapidly to plateaus at the early stage of measurement for all the experimental groups caused by the formation of RBC aggregates. The DIDS-treated group exhibits the lowest echogenicity level due to the inhibitory effect of DIDS on RBC aggregation. The correlation analysis between consecutive speckle patterns seems to be useful to examine the variation of blood fluidity and the progress of clot formation. Whole blood coagulation is observed to be accelerated by DIDS treatment. In addition, the results of skewness and kurtosis analysis indicated that RBC aggregates may be disrupted during blood coagulation. The present study suggests that HFUS has good potential for simultaneous monitoring of RBC aggregation and blood coagulation to examine the relationship between them.
本研究旨在探讨高频超声（HFUS）用于同时监测血液凝固和红细胞（RBC）聚集的可行性。使用 35MHz 超声扫描仪，从包括 4,4'-二异硫氰酸基二苯乙烯-2,2'-二磺酸（DIDS）处理、非凝固和正常对照组的三组大鼠全血样本中获取超声散斑数据。计算了血液回声强度的变化、散斑强度概率分布的形状参数（偏度和峰度）以及两个连续散斑数据之间的相关系数，作为从采血后立即开始随时间的变化函数。由于 RBC 聚集的形成，所有实验组在测量的早期阶段，血液回声强度迅速增加到平台期。由于 DIDS 对 RBC 聚集的抑制作用，DIDS 处理组表现出最低的回声强度水平。连续散斑模式之间的相关分析似乎有助于检查血液流动性的变化和凝血形成的进展。全血凝固被观察到通过 DIDS 处理而加速。此外，偏度和峰度分析的结果表明，在血液凝固过程中 RBC 聚集可能会被破坏。本研究表明，HFUS 具有同时监测 RBC 聚集和血液凝固的良好潜力，以检查它们之间的关系。
