Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
Bioorg Med Chem. 2012 Mar 15;20(6):1990-2001. doi: 10.1016/j.bmc.2011.12.022. Epub 2011 Dec 21.
Elucidating the mechanism of action of bioactive compounds, such as commonly used pharmaceutical drugs and biologically active natural products, in the cells and the living body is important in drug discovery research. To this end, isolation and identification of target protein(s) for the bioactive compound are essential in understanding its function fully. And, development of reliable tools and methodologies capable of addressing efficiently identification and characterization of the target proteins based on the bioactive compounds accelerates drug discovery research. Affinity-based isolation and identification of target molecules for the bioactive compounds is a classic, but still powerful approach. This paper introduces recent progress on affinity chromatography system, focusing on development of practical affinity matrices and useful affinity-based methodologies on target identification. Beneficial affinity chromatography systems with using practical tools and useful methodologies facilitate chemical biology and drug discovery research.
阐明生物活性化合物（如常用的药物和具有生物活性的天然产物）在细胞和生物体中的作用机制，在药物发现研究中非常重要。为此，对于生物活性化合物，分离和鉴定其靶蛋白是全面了解其功能的关键。并且，开发能够基于生物活性化合物有效鉴定和表征靶蛋白的可靠工具和方法学，也可以加速药物发现研究。基于亲和性的生物活性化合物靶分子的分离和鉴定是一种经典但仍然强大的方法。本文介绍了亲和色谱系统的最新进展，重点介绍了实用亲和配基和用于靶标鉴定的有用亲和方法的开发。使用实用工具和有用方法学的有益亲和色谱系统，有助于化学生物学和药物发现研究。
