Department of Histopathology and Cytology, University of Khartoum, Khartoum, Sudan.
Clin Lymphoma Myeloma Leuk. 2012 Apr;12(2):127-31. doi: 10.1016/j.clml.2011.11.006. Epub 2012 Jan 20.
Results of several studies have suggested a probable etiologic association between Epstein-Barr virus (EBV) and leukemias; therefore, the aim of this study was to investigate the association of EBV in childhood leukemia.
A direct isothermal amplification method was developed for detection of the latent membrane protein 1 (LMP1) of EBV in the peripheral blood of 80 patients with leukemia (54 had lymphoid leukemia and 26 had myeloid leukemia) and of 20 hematologically healthy control subjects.
EBV LMP1 gene transcripts were found in 29 (36.3%) of the 80 patients with leukemia but in none of the healthy controls (P < .0001). Of the 29 EBV(+) cases, 23 (79.3%), 5 (17.3%), and 1 (3.4%) were acute lymphoblastic leukemia, acute myeloid leukemia, and chronic myeloid leukemia, respectively.
EBV LMP1 gene transcriptional activity was observed in a significant proportion of patients with acute lymphoblastic leukemia. EBV infection in patients with lymphoid leukemia may be a factor involved in the high incidence of pediatric leukemia in the Sudan.
多项研究的结果表明，EB 病毒（EBV）与白血病之间可能存在病因关联；因此，本研究旨在探讨 EBV 与儿童白血病的关联。
本研究开发了一种直接等温扩增方法，用于检测 80 例白血病患者（54 例为淋巴细胞白血病，26 例为髓系白血病）和 20 例血液学健康对照者外周血中的 EBV 潜伏膜蛋白 1（LMP1）。
在 80 例白血病患者中有 29 例（36.3%）发现 EBV LMP1 基因转录本，但在健康对照组中无一例发现（P<0.0001）。在 29 例 EBV(+)病例中，分别有 23 例（79.3%）、5 例（17.3%）和 1 例（3.4%）为急性淋巴细胞白血病、急性髓细胞白血病和慢性髓细胞白血病。
在相当一部分急性淋巴细胞白血病患者中观察到 EBV LMP1 基因转录活性。EBV 感染可能是苏丹儿童白血病高发的一个因素。
Zotero 插件