苏丹鼻咽癌（NPC）患者中爱泼斯坦-巴尔病毒（EBV）的分子筛查。
Molecular screening for Epstein Barr virus (EBV) among Sudanese patients with nasopharyngeal carcinoma (NPC).
作者信息
Ahmed Hussain Gadelkarim, Suliman Rania Saad Abdul Gader, El Aziz Mohammed Siddig Abd, Alshammari Fawaz D
机构信息
Department of Pathology, College of Medicine, Molecular Diagnostics and Personalized Therapeutics Unit, University of Hail, Hail, Kingdom of Saudi Arabia ; Department of Histopathology and Cytology, FMLS, University of Khartoum, Hail, Sudan.
Department of Histopathology and Cytology, Faculty of Medical Laboratory Science, Sudan University for Science and Technology, Khartoum, Sudan.
出版信息
Infect Agent Cancer. 2015 Feb 17;10:6. doi: 10.1186/s13027-015-0002-4. eCollection 2015.
OBJECTIVE
The aim of this study was to screen for the presence of Epstein Barr Virus (EBV) among Sudanese patients with Nasopharyngeal Carcinoma (NPC).
METHODS
In this study, 150 tissue samples that were previously diagnosed as having NPC were screened for the presence of EBV using Polymerase Chain Reaction (PCR). PCR was performed to amplify two viral genes; EBV nuclear antigen-4 (EBNA-4) and latent membrane protein-1 (LMP1).
RESULTS
EBV genes were detected in 92/150 (61.3%) tissue samples. Of the 92 infected samples, 58/92 (63%) were found among males and 34/92 (37%) were among females.
CONCLUSION
EBV is prevalent in the Sudan and responsible of the vast majority of cases of NPC.
目的
本研究旨在筛查苏丹鼻咽癌（NPC）患者中是否存在爱泼斯坦-巴尔病毒（EBV）。
方法
在本研究中，使用聚合酶链反应（PCR）对150份先前诊断为患有NPC的组织样本进行EBV检测。进行PCR以扩增两个病毒基因；EBV核抗原-4（EBNA-4）和潜伏膜蛋白-1（LMP1）。
结果
在150份组织样本中的92份（61.3%）检测到EBV基因。在92份受感染样本中，58/92（63%）为男性，34/92（37%）为女性。
结论
EBV在苏丹流行，并且是绝大多数NPC病例的病因。
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