D'Andrea P, Grandolfo M, de Bernard B, Vittur F
Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Trieste, Italy.
Exp Cell Res. 1990 Nov;191(1):22-6. doi: 10.1016/0014-4827(90)90030-e.
The differentiation of preosseous chondrocytes begins with the proliferation of resting cells and results in the expression of the hypertrophic phenotype. The effect of fetal calf serum on chondrocyte mitogenesis and intracellular Ca2+ concentration was studied in resting and hypertrophic cells in primary culture. Resting chondrocytes respond to the growth stimulus with immediate release of Ca2+ from intracellular stores and with opening of the plasma membrane Ca2+ channels. These events may be related to the elevated [3H]thymidine incorporation observed after serum exposure. In contrast, in hypertrophic chondrocytes the lower rate of DNA synthesis seems to be coupled with a lower activity of the Ca2+ signaling mechanism and, probably, with reduced intracellular calcium stores. It is proposed that expression of the Ca2+ signaling mechanism may be modulated during the differentiation of preosseous chondrocytes.
骨前软骨细胞的分化始于静止细胞的增殖，并导致肥大表型的表达。研究了胎牛血清对原代培养的静止和肥大细胞中软骨细胞有丝分裂及细胞内Ca2+浓度的影响。静止软骨细胞对生长刺激的反应是立即从细胞内储存中释放Ca2+并打开质膜Ca2+通道。这些事件可能与血清暴露后观察到的[3H]胸腺嘧啶核苷掺入增加有关。相反，在肥大软骨细胞中，较低的DNA合成速率似乎与Ca2+信号传导机制的较低活性以及可能与细胞内钙储存减少有关。有人提出，在骨前软骨细胞分化过程中，Ca2+信号传导机制的表达可能受到调节。
