Wolffing B K, Gabridge M G
In Vitro. 1979 Apr;15(4):308-14. doi: 10.1007/BF02618956.
Exogenous cyclic AMP and dibutyryl cyclic AMP decreased the relative ciliary activity values of tracheal organ cultures. In contrast, theophylline and cholera toxin were not ciliostatic. The use of a radioimmunoassay for cyclic AMP indicated that all of the tested substances increased intracellular cyclic AMP levels to some extent (from 3-fold for cholera toxin to almost 40-fold for dibutyryl cyclic AMP). Physical inactivation of explants by either freeze-thaw or heat destroyed all ciliary activity and greatly decreased intracellular cyclic AMP levels. Cyclic AMP levels of explants remained relatively constant during in vitro cultivation. Three strains of Mycoplasma pneumoniae were found to contain extremely low amounts of cyclic AMP. Infection of tracheal explants produced a significant decrease in relative ciliary activity, but only a slight decline in organ-culture cyclic AMP levels.
外源性环磷酸腺苷（cAMP）和二丁酰环磷酸腺苷降低了气管器官培养物的相对纤毛活性值。相比之下，茶碱和霍乱毒素没有抑制纤毛运动的作用。采用放射免疫分析法检测环磷酸腺苷表明，所有受试物质均能在一定程度上提高细胞内环磷酸腺苷水平（霍乱毒素提高3倍，二丁酰环磷酸腺苷几乎提高40倍）。通过冻融或加热对外植体进行物理灭活会破坏所有纤毛活性，并大幅降低细胞内环磷酸腺苷水平。外植体的环磷酸腺苷水平在体外培养过程中保持相对稳定。发现三株肺炎支原体所含的环磷酸腺苷量极低。气管外植体感染后，相对纤毛活性显著降低，但器官培养物中的环磷酸腺苷水平仅略有下降。
