Petrenko Yu A, Ivanov R V, Lozinsky V I, Petrenko A Yu
Institute of Cryobiology and Cryomedicine Problems, National Academy of Sciences of Ukraine, Khar'kov.
Bull Exp Biol Med. 2011 Feb;150(4):543-6. doi: 10.1007/s10517-011-1185-3.
We performed a comparative study of the localization, distribution, metabolic activity, and surface properties of human bone marrow mesenchymal stromal cells after static and perfusion seeding to macroporous alginate cryogels. A simple perfusion system for mesenchymal stromal cell seeding to macroporous alginate cryogel sponges proposed in this study resulted in rapid and uniform distribution of cells within the whole volume of the scaffold preserving functional and morphological properties of the cells.
我们对人骨髓间充质基质细胞在静态接种和灌注接种到大孔海藻酸盐冷冻凝胶后的定位、分布、代谢活性及表面特性进行了比较研究。本研究提出的一种用于将间充质基质细胞接种到大孔海藻酸盐冷冻凝胶海绵上的简单灌注系统，可使细胞在支架的整个体积内快速且均匀地分布，同时保持细胞的功能和形态特性。
