Lehmacher A, Vogt A B, Hensel R
Max-Planck-Institut für Biochemie, Martinsried, FRG.
FEBS Lett. 1990 Oct 15;272(1-2):94-8. doi: 10.1016/0014-5793(90)80456-s.
Starting from 2-phosphoglycerate the biosynthesis of cDPG comprises two steps: (i) the phosphorylation of 2-phosphoglycerate to 2,3-diphosphoglycerate and (ii) the intramolecular cyclization to cyclic 2,3-diphosphoglycerate. The involved enzymes, 2-phosphoglycerate kinase and cyclic 2,3-diphosphoglycerate synthetase, were purified form Methanothermus fervidus. Their molecular and catalytic properties were characterized.
从2-磷酸甘油酸开始,环二磷酸甘油(cDPG)的生物合成包括两个步骤:(i)2-磷酸甘油酸磷酸化生成2,3-二磷酸甘油酸,以及(ii)分子内环化生成环2,3-二磷酸甘油酸。所涉及的酶,即2-磷酸甘油酸激酶和环2,3-二磷酸甘油酸合成酶,是从嗜热甲烷栖热菌中纯化得到的。对它们的分子和催化特性进行了表征。