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环2,3-二磷酸甘油酸的生物合成。嗜热甲烷栖热菌中2-磷酸甘油酸激酶和环2,3-二磷酸甘油酸合成酶的分离与鉴定。

Biosynthesis of cyclic 2,3-diphosphoglycerate. Isolation and characterization of 2-phosphoglycerate kinase and cyclic 2,3-diphosphoglycerate synthetase from Methanothermus fervidus.

作者信息

Lehmacher A, Vogt A B, Hensel R

机构信息

Max-Planck-Institut für Biochemie, Martinsried, FRG.

出版信息

FEBS Lett. 1990 Oct 15;272(1-2):94-8. doi: 10.1016/0014-5793(90)80456-s.

DOI:10.1016/0014-5793(90)80456-s
PMID:2226838
Abstract

Starting from 2-phosphoglycerate the biosynthesis of cDPG comprises two steps: (i) the phosphorylation of 2-phosphoglycerate to 2,3-diphosphoglycerate and (ii) the intramolecular cyclization to cyclic 2,3-diphosphoglycerate. The involved enzymes, 2-phosphoglycerate kinase and cyclic 2,3-diphosphoglycerate synthetase, were purified form Methanothermus fervidus. Their molecular and catalytic properties were characterized.

摘要

从2-磷酸甘油酸开始,环二磷酸甘油(cDPG)的生物合成包括两个步骤:(i)2-磷酸甘油酸磷酸化生成2,3-二磷酸甘油酸,以及(ii)分子内环化生成环2,3-二磷酸甘油酸。所涉及的酶,即2-磷酸甘油酸激酶和环2,3-二磷酸甘油酸合成酶,是从嗜热甲烷栖热菌中纯化得到的。对它们的分子和催化特性进行了表征。

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