Department of Pharmacology, Chung Shan Medical University, 110 Sec. 1 Chien-Kuo N. Road, Taichung, Taiwan.
Int Endod J. 2012 Jul;45(7):619-26. doi: 10.1111/j.1365-2591.2012.02017.x. Epub 2012 Jan 23.
To evaluate the expression of tumour necrosis factor-α and surface antigens by bisphenol A-glycidyl-methacrylate (BisGMA) on murine macrophage cell line RAW264.7.
Cytotoxicity was measured by tetrazolium bromide reduction assay. Tumour necrosis factor (TNF)-α was analysed by enzyme-linked immunosorbent assay. Cell surface antigens were investigated by flowcytometry. Statistical analyses were performed using anova followed by the Bonferroni's t-test for multigroup comparisons.
BisGMA exhibited cytotoxicity to RAW264.7 in a dose-dependent manner (P < 0.05) during 2-h incubation period. BisGMA was found to increase TNF-α secretion in a dose-dependent manner (P < 0.05). In addition, CD11, CD14, CD45, CD54, CD40, CD80, and MHC II were significantly stimulated by BisGMA in a dose-dependent manner (P < 0.05). However, MHC I expression was not affected by BisGMA (P > 0.05).
Taken together, the ability of macrophages to induce an appropriate immune response when exposed to BisGMA has the potential to upregulate TNF-α production and expression of surface antigens.
评估双酚 A 缩水甘油基甲基丙烯酸酯（BisGMA）对鼠源巨噬细胞 RAW264.7 表面抗原和肿瘤坏死因子-α（TNF-α）表达的影响。
通过噻唑蓝（MTT）比色法检测细胞毒性。酶联免疫吸附法（ELISA）检测 TNF-α的分泌。流式细胞术检测细胞表面抗原。采用方差分析（ANOVA）和 Bonferroni 检验进行多组间比较。
BisGMA 在孵育 2 小时内呈剂量依赖性地表现出对 RAW264.7 的细胞毒性（P < 0.05）。BisGMA 呈剂量依赖性地增加 TNF-α的分泌（P < 0.05）。此外，CD11、CD14、CD45、CD54、CD40、CD80 和 MHC II 的表达也呈剂量依赖性地被 BisGMA 刺激（P < 0.05）。然而，BisGMA 对 MHC I 的表达没有影响（P > 0.05）。
综上所述，巨噬细胞在接触 BisGMA 时诱导适当免疫反应的能力可能会增加 TNF-α的产生和表面抗原的表达。
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