Oh Chang Seok, Lee Sang Jun, Park Jun Bum, Lee Soong Deok, Seo Seung Bum, Kim Hye Yeon, Kim Jaehyup, Kim Yi-Suk, Shin Dong Hoon
Institute of Forensic Medicine, Seoul National University College of Medicine, 28, Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
J Forensic Sci. 2012 May;57(3):820-5. doi: 10.1111/j.1556-4029.2011.02044.x. Epub 2012 Jan 23.
Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFℓSTR(®) MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFℓSTR(®) MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger-sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large-sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples.
多重常染色体短串联重复序列（STR）基因分型使研究人员能够从古代人类样本中获取遗传信息。在本研究中，我们使用从韩国中世纪古墓收集的人类股骨（n = 8），测试了新开发的用于古代DNA（aDNA）常染色体STR分析的AmpFℓSTR(®) MiniFiler™试剂盒。从骨骼中提取aDNA后，使用AmpFℓSTR(®) MiniFiler™和Identifiler™试剂盒对每个样本重复进行常染色体STR分析。使用Identifiler™试剂盒仅能获得21.87%的较大位点图谱，而MiniFiler™试剂盒分析确定了相同位点图谱的75%。从高度降解的aDNA中成功扩增出大型STR标记表明，MiniFiler™试剂盒可能是古代样本常规STR试剂盒分析的有用补充。
