Mulero Julio J, Chang Chien Wei, Lagacé Robert E, Wang Dennis Y, Bas Jennifer L, McMahon Timothy P, Hennessy Lori K
Applied Biosystems, 850 Lincoln Centre Dr., Foster City, CA 94404, USA.
J Forensic Sci. 2008 Jul;53(4):838-52. doi: 10.1111/j.1556-4029.2008.00760.x.
DNA typing of degraded DNA samples can be a challenging task when using the current commercially available multiplex short tandem repeat (STR) analysis kits. However, the ability to type degraded DNA specimens improves by redesigning current STR marker amplicons such that smaller sized polymerase chain reaction (PCR) products are generated. In an effort to increase the amount of information derived from these types of DNA samples, the AmpFlSTR MiniFiler PCR Amplification Kit has been developed. The kit contains reagents for the amplification of eight miniSTRs which are the largest sized loci in the AmpFlSTR Identifiler PCR Amplification Kit (D7S820, D13S317, D16S539, D21S11, D2S1338, D18S51, CSF1PO, and FGA). Five of these STR loci (D16S539, D21S11, D2S1338, D18S51, and FGA) also are some of the largest loci in the AmpFlSTR SGM Plus kit. This informative nine-locus multiplex, which includes the gender-identification locus Amelogenin, has been validated according to the FBI/National Standards and SWGDAM guidelines. Our results demonstrate significant performance improvements in models of DNA degradation, PCR inhibition, and nonprobative samples when compared to the AmpFlSTR Identifiler and SGM Plus kits. These data support that the MiniFiler kit will increase the likelihood of obtaining additional STR information from forensic samples in situations in which standard STR chemistries fail to produce complete profiles.
使用当前市售的多重短串联重复序列（STR）分析试剂盒对降解的DNA样本进行DNA分型可能是一项具有挑战性的任务。然而，通过重新设计当前的STR标记扩增子，使得产生更小尺寸的聚合酶链反应（PCR）产物，对降解DNA样本进行分型的能力得到了提高。为了增加从这类DNA样本中获得的信息量，已开发出AmpFlSTR MiniFiler PCR扩增试剂盒。该试剂盒包含用于扩增八个微型STR的试剂，这些微型STR是AmpFlSTR Identifiler PCR扩增试剂盒中尺寸最大的基因座（D7S820、D13S317、D16S539、D21S11、D2S1338、D18S51、CSF1PO和FGA）。这些STR基因座中的五个（D16S539、D21S11、D2S1338、D18S51和FGA）也是AmpFlSTR SGM Plus试剂盒中一些尺寸较大的基因座。这个包含性别鉴定基因座Amelogenin的信息丰富的九基因座多重检测体系已根据联邦调查局/国家标准和SWGDAM指南进行了验证。我们的结果表明，与AmpFlSTR Identifiler和SGM Plus试剂盒相比，在DNA降解、PCR抑制和无证据样本模型中，性能有显著提高。这些数据支持，在标准STR化学方法无法产生完整图谱的情况下，MiniFiler试剂盒将增加从法医样本中获得额外STR信息的可能性。
