Fusi F, Bronson R A
Department of Obstetrics and Gynecology, Health Science Center-State University of New York (SUNY), Stony Brook 11794-8091.
Fertil Steril. 1990 Nov;54(5):887-93. doi: 10.1016/s0015-0282(16)53951-x.
Sperm reside within the female reproductive tract before the occurrence of fertilization. During this time they undergo surface modifications associated with changes in their functional state. To study their antigenic expression, capacitated and acrosome-reacted sperm were incubated with sera that had previously been tested for antisperm antibodies against fresh washed sperm, as detected by indirect immunobead binding. Forty-eight percent of previously positive and 20% of previously negative sera reacted differently with sperm after an extended time (18 hours) of incubation in serum or after sperm capacitation. These results suggest that current techniques of antisperm antibodies detection be modified to include testing sera after prolonged incubation times with both capacitated as well as fresh sperm.
在受精发生之前，精子存在于女性生殖道内。在此期间，它们会经历与功能状态变化相关的表面修饰。为了研究它们的抗原表达，将获能和顶体反应的精子与之前针对新鲜洗涤过的精子检测过抗精子抗体的血清一起孵育，通过间接免疫珠结合检测。在血清中延长孵育时间（18小时）后或精子获能后，48%之前呈阳性的血清和20%之前呈阴性的血清与精子的反应有所不同。这些结果表明，当前抗精子抗体检测技术应进行改进，以包括在用获能精子以及新鲜精子长时间孵育后检测血清。
