Thakur Asmitananda, Hui Ren, Hongyan Zhang, Tian Yang, Tianjun Chen, Mingwei Chen
Department of Respiratory Medicine, The First Affiliated Hospital of Xian Jiaotong University, School of Medicine, Xian-710 061, People's Republic of China.
J Cancer Res Ther. 2011 Oct-Dec;7(4):421-6. doi: 10.4103/0973-1482.92007.
Paecilomyces hepiali (PH) is a derivative of Cordyceps sinensis (CS), a fungus that has been shown to have anti-cancer and pro-apoptotic effects. Here, we aimed to investigate the effect of in vitro PH treatment on cell proliferation, cell cycling, apoptosis, and tumor necrosis factor-alfa (TNF-α) mRNA expression in human lung adenocarcinoma A549 cells (A549).
A549 cells were treated with an aqueous extract of PH at concentrations of 0.25, 0.5, 1, 2, and 4 mg/ml. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate cellular viability and proliferation, while flow cytometry (FCM) was used to examine cell cycling. Apoptosis was assayed using Annexin V Fluorescein Isothiocyanate / Propidium Iodide (V-FITC/PI) and FCM. TNF-α mRNA expression was measured with reverse transcriptase-polymerase chain reaction (RT-PCR).
Cell proliferation was significantly suppressed by treatment with 1, 2, and 4 mg/ml of PH extract. Furthermore, the MTT assay showed that cell proliferation was inhibited in a concentration-time-dependent manner. As the concentration of the PH treatment increased, there were fewer cells in the S phase, and more cells in the G0/G1 and G2 phases. After 24 h of treatment, apoptosis was induced by a dose of 2 mg/ml of PH. TNF-α mRNA expression was significantly higher in the intervention groups and was positively associated with treatment concentration.
These results indicate that in vitro treatment with an aqueous extract from PH limits cell proliferation, induces apoptosis, and causes cell cycle arrest of A549 cells; this suggests that it may have potential as a therapy for lung adenocarcinoma.
蝙蝠蛾拟青霉（PH）是冬虫夏草（CS）的衍生物，冬虫夏草已被证明具有抗癌和促凋亡作用。在此，我们旨在研究体外PH处理对人肺腺癌A549细胞（A549）的细胞增殖、细胞周期、凋亡和肿瘤坏死因子-α（TNF-α）mRNA表达的影响。
用浓度为0.25、0.5、1、2和4mg/ml的PH水提取物处理A549细胞。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法评估细胞活力和增殖，同时采用流式细胞术（FCM）检测细胞周期。使用膜联蛋白V异硫氰酸荧光素/碘化丙啶（V-FITC/PI）和FCM检测凋亡。用逆转录聚合酶链反应（RT-PCR）测量TNF-αmRNA表达。
用1、2和4mg/ml的PH提取物处理可显著抑制细胞增殖。此外，MTT法显示细胞增殖呈浓度-时间依赖性抑制。随着PH处理浓度的增加，S期细胞减少，G0/G1期和G2期细胞增多。处理24小时后，2mg/ml的PH剂量可诱导凋亡。干预组TNF-αmRNA表达显著更高，且与处理浓度呈正相关。
这些结果表明，体外使用PH水提取物处理可限制A549细胞的增殖，诱导凋亡并导致细胞周期停滞；这表明它可能具有作为肺腺癌治疗方法的潜力。
