Division of General Thoracic Surgery, University Hospital Berne, Berne CH-3010, Switzerland.
Anticancer Res. 2013 Dec;33(12):5365-73.
Resistance to chemotherapy in lung adenocarcinoma remains a major obstacle. We examined the potential role of Octamer-binding transcription factor-4B (OCT4B) in enhancing sensitivity of lung adenocarcinoma cells to cisplatin.
RNAi interference was used to examine the role of OCT4B in cisplatin-treated A549 cells. Cells were transfected with OCT4B siRNA prior to a 48-h cisplatin treatment. Propidium iodide (PI) and caspase-3 staining were used to determine cell viability and apoptosis. Cell-cycle analysis was performed to evaluate alterations in phase distribution.
OCT4B suppression in cells increased the number of non-viable, PI(+), and apoptotic, caspase-3(+) cells in the presence and absence of cisplatin treatment. Importantly, cisplatin treatment of OCT4B-suppressed cells resulted in a marked transition of cells from G0/G1 to G2/M phase.
Silencing of OCT4B confers sensitivity to cisplatin treatment in A549 cells via cell-cycle regulation, increased proliferation and enhancement of cisplatin-induced apoptosis. OCT4B clearly protects A549 cells from apoptosis.
肺腺癌对化疗的耐药性仍然是一个主要障碍。我们研究了 Octamer-binding transcription factor-4B（OCT4B）在增强肺腺癌细胞对顺铂敏感性中的潜在作用。
使用 RNAi 干扰来研究 OCT4B 在顺铂处理的 A549 细胞中的作用。在进行 48 小时顺铂处理之前，用 OCT4B siRNA 转染细胞。碘化丙啶（PI）和 caspase-3 染色用于确定细胞活力和细胞凋亡。细胞周期分析用于评估相分布的变化。
细胞中 OCT4B 的抑制增加了顺铂处理存在和不存在时非存活、PI(+)和凋亡、caspase-3(+)细胞的数量。重要的是，OCT4B 沉默的细胞在顺铂处理后从 G0/G1 期明显过渡到 G2/M 期。
沉默 OCT4B 通过细胞周期调控、增强增殖和增强顺铂诱导的细胞凋亡，使 A549 细胞对顺铂治疗敏感。OCT4B 明显保护 A549 细胞免受凋亡。
