Bossert N L, Nelson K G, Ross K A, Takahashi T, McLachlan J A
Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
Dev Biol. 1990 Nov;142(1):75-85. doi: 10.1016/0012-1606(90)90152-9.
The ontogeny of the epidermal growth factor (EGF) receptor in the different cell types in the neonatal and immature mouse uterus and vagina was examined. Immunohistochemical examination of prenatal and neonatal reproductive tracts with a polyclonal antibody to the EGF receptor shows immunoreactive EGF receptors as early as Day 13 of gestation. Autoradiographic analysis of tissue sections at 3 to 17 days of age (the day of birth is Day 1) demonstrates that both uterine and vaginal epithelial and stromal cells are capable of binding 125I-labeled EGF. Both the 125I-labeled EGF autoradiography and immunohistochemistry in whole tissue show higher EGF receptor levels in the uterine epithelium than the uterine stroma. The presence of EGF receptors was also confirmed by affinity labeling and Scatchard analysis of isolated uterine cell types at 7 and/or 17 days of age. However, in contrast to the autoradiography and immunohistochemistry data of intact tissue, the affinity labeling and Scatchard data of isolated cells indicate that the uterine stroma contains higher levels of EGF receptor than that of the uterine epithelium. The reason for this discrepancy between the different techniques is, as yet, unknown. Regardless of the differences in the actual numbers of EGF receptors obtained, our data demonstrate that the developing mouse reproductive tract contains immunoreactive EGF receptors that are capable of binding 125I-labeled EGF.
研究了新生和未成熟小鼠子宫及阴道中不同细胞类型的表皮生长因子（EGF）受体的个体发生。用针对EGF受体的多克隆抗体对产前和新生生殖道进行免疫组织化学检查显示，早在妊娠第13天就有免疫反应性EGF受体。对3至17日龄（出生日为第1天）的组织切片进行放射自显影分析表明，子宫和阴道的上皮及基质细胞均能够结合125I标记的EGF。全组织的125I标记EGF放射自显影和免疫组织化学均显示，子宫上皮中的EGF受体水平高于子宫基质。通过对7日龄和/或17日龄分离的子宫细胞类型进行亲和标记和Scatchard分析，也证实了EGF受体的存在。然而，与完整组织的放射自显影和免疫组织化学数据相反，分离细胞的亲和标记和Scatchard数据表明，子宫基质中的EGF受体水平高于子宫上皮。不同技术之间这种差异的原因尚不清楚。尽管获得的EGF受体实际数量存在差异，但我们的数据表明，发育中的小鼠生殖道含有能够结合125I标记EGF的免疫反应性EGF受体。
