Supon P, Stecha P, Haskins K
Barbara Davis Center for Childhood Diabetes, University of Colorado Health Sciences Center, Denver 80262.
Diabetes. 1990 Nov;39(11):1366-72. doi: 10.2337/diab.39.11.1366.
We produced a panel of monoclonal autoantibodies from the spleen cells of prediabetic nonobese diabetic mice. The antibodies were selected on the basis of their ability to bind to the surface of nondiabetic mouse islet cells, and from greater than 4000 hybridomas screened, 35 islet-reactive antibodies were isolated. Most of these reagents also bind to nondiabetic rat and human islet cells and beta-cell tumor lines. A few of the antibodies were found to be reactive with insulin. When tested for cross-reactivity with other cell types, most of the antibodies were found to be islet specific.
我们从糖尿病前期非肥胖糖尿病小鼠的脾细胞中制备了一组单克隆自身抗体。这些抗体是根据它们与非糖尿病小鼠胰岛细胞表面结合的能力来选择的，在筛选的4000多个杂交瘤中，分离出了35种胰岛反应性抗体。这些试剂中的大多数也与非糖尿病大鼠和人类胰岛细胞以及β细胞肿瘤系结合。发现其中一些抗体与胰岛素有反应。当测试与其他细胞类型的交叉反应性时，发现大多数抗体具有胰岛特异性。
