鉴定稳定蛋白降解的疏水区标签。
Identification of hydrophobic tags for the degradation of stabilized proteins.
机构信息
Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511, USA.
出版信息
Chembiochem. 2012 Mar 5;13(4):538-41. doi: 10.1002/cbic.201100793. Epub 2012 Jan 23.
Abstract
New HyTs are a knockout: we previously reported that labeling HaloTag proteins with low molecular weight hydrophobic tags (HyTs) leads to targeted degradation of HaloTag fusion proteins. In this report, we employed a chemical approach to extend this hydrophobic tagging methodology to highly stabilized proteins by synthesizing and evaluating a library of HyTs, which led to the identification of HyT36.
摘要
新型 HyTs 是一种有效的方法：我们之前报道过，通过将低分子量疏水性标签（HyTs）标记到 HaloTag 蛋白上，可导致 HaloTag 融合蛋白的靶向降解。在本报告中，我们通过合成和评估一系列 HyTs 文库，采用一种化学方法将这种疏水性标记方法扩展到高度稳定的蛋白质，从而鉴定出了 HyT36。
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