BASF Beauty Care Solutions France SAS, Laboratoires Sérobiologiques, Essey-lès-Nancy Cedex, France.
Skin Res Technol. 2013 Feb;19(1):e1-12. doi: 10.1111/j.1600-0846.2011.00599.x. Epub 2012 Jan 24.
The confocal laser scanning microscope allows performing acquisition of several histological sections with precise visual morphological landmarks and their reconstruction. A powerful and modern confocal microscope enables to quickly reconstruct virtual 3D models.
The main goal was to develop a new platform to reconstruct complex mosaic serial data, interact with it in an immersive 3D environment, and give to the observers a feeling of 'presence' inside the skin.
We have developed novel methods that transform the data into alternative representation, well-suited to explore cutaneous structures in detail and to observe fields of data from different points of view. This new way of data reconstruction in volume requires optimization of intensities, automatic matching algorithms and depth alignment.
The new platform - SkinExplorer evolves as a 3D exploration prototype. This technology provides an immersive virtual environment to explore cutaneous microstructures. Several serial histological sections can be matched by stacks, aligned in depth by sections and merged together to be visualized as a whole. All these time-consuming steps have been dramatically speed-up using rapid image processing. The advantages of using virtual reality technologies such as the ones used in the SkinExplorer platform are automatic matching, precise alignment, better data perception, lower memory requirement, and higher quantity of simultaneously displayed data. This platform can render volumetric data and isosurfaces, separately or both at the same time. Lighting and depth perception are enhanced using 'Sphere Mapping', 'Ambient Occlusion', and 'Halo' methods when displaying iso-surfacic volume models with high complexity depth. The assets of the platform are to interpret complex three-dimensional data, to observe and explore 3D virtual models, and to show effects of cosmetic treatments.
共聚焦激光扫描显微镜允许对具有精确视觉形态学标志的几个组织切片进行采集，并对其进行重建。强大而现代的共聚焦显微镜能够快速重建虚拟 3D 模型。
主要目标是开发一个新的平台，用于重建复杂的镶嵌序列数据，在沉浸式 3D 环境中与数据进行交互，并使观察者能够感受到皮肤内部的“存在”。
我们开发了新的方法，将数据转换为替代表示形式，非常适合详细探索皮肤结构，并从不同的角度观察数据场。这种体积数据的新重建方式需要对强度进行优化，使用自动匹配算法和深度对齐。
新平台-SkinExplorer 作为 3D 探索原型不断发展。该技术提供了一个沉浸式的虚拟环境，用于探索皮肤微观结构。可以通过堆栈匹配多个连续的组织切片，通过切片进行深度对齐，并将它们合并在一起作为一个整体进行可视化。所有这些耗时的步骤都通过快速图像处理得到了显著加速。使用虚拟现实现技术（如 SkinExplorer 平台中使用的技术）的优点是自动匹配、精确对齐、更好的数据感知、更低的内存要求和更高的同时显示数据量。该平台可以渲染体积数据和等位面，分别或同时显示。在显示具有高复杂度深度的等位面体模型时，使用“球体映射”、“环境遮挡”和“晕影”方法增强照明和深度感知。该平台的优势在于解释复杂的三维数据，观察和探索 3D 虚拟模型，并展示美容治疗的效果。
