Research Center for Environmental Genomics, Graduate School of Science and Technology, Kobe University, Kobe, Hyogo, Japan.
J Agric Food Chem. 2012 Feb 22;60(7):1605-12. doi: 10.1021/jf203585b. Epub 2012 Feb 8.
Coplanar polychlorinated biphenyls (Co-PCBs) consisting of non-ortho and mono-ortho-chlorinated PCBs are dioxin-like compounds and cause wide contamination in the environment. To monitor Co-PCB residues, it was attempted to establish an enzyme-linked immunosorbent assay (ELISA) with monoclonal and recombinant antibodies selective to Co-PCBs. When 3,3',5,5'-tetrachlorobiphenoxybutyric acid (PCBH)-keyhole limpet hemocyanin conjugate was immunized into mice, two monoclonal antibodies, Mab-0217 and Mab-4444, were obtained. 3,3',5,5'-Tetrachlorobiphenyl (PCB80) was determined with an IC(50) value of 2.6 and 0.46 ng mL(-1) in ELISA based on Mab-0217 and Mab-4444, respectively. Mab-4444 cross-reacted with Co-PCB congeners, except for PCB77 and PCB81. Mab-0217 reacted with PCB80 and cross-reacted with PCB111. A single-chain variable fragment (scFv) antibody derived from Mab-4444 was produced in recombinant Escherichia coli cells. The scFv antibody showed nearly the same sensitivity toward PCBH as the parent monoclonal antibody in ELISA. These results clearly suggested that Mab-4444 and its scFv antibodies were suitable for monitoring the representative congeners of Co-PCBs.
共平面多氯联苯（Co-PCBs）由非邻位和单邻位氯代 PCBs 组成，属于类二恶英化合物，会对环境造成广泛污染。为了监测 Co-PCB 残留，尝试使用对 Co-PCBs 具有选择性的单克隆抗体和重组抗体建立酶联免疫吸附测定（ELISA）。当 3,3',5,5'-四氯联苯氧基丁酸（PCBH）-钥孔蓝蛋白缀合物被免疫到小鼠中时，获得了两种单克隆抗体，Mab-0217 和 Mab-4444。基于 Mab-0217 和 Mab-4444，ELISA 中分别确定 3,3',5,5'-四氯联苯（PCB80）的 IC50 值为 2.6 和 0.46ngmL-1。Mab-4444 与 Co-PCB 同系物交叉反应，除了 PCB77 和 PCB81。Mab-0217 与 PCB80 反应，并与 PCB111 交叉反应。从 Mab-4444 中产生的单链可变片段（scFv）抗体在重组大肠杆菌细胞中生产。scFv 抗体在 ELISA 中对 PCBH 的敏感性与亲本单克隆抗体几乎相同。这些结果清楚地表明，Mab-4444 及其 scFv 抗体适用于监测 Co-PCBs 的代表性同系物。
