Black G W, Lyons C M, Williams E, Colby J, Kehoe M, O'Reilly C
School of Biology, Sunderland Polytechnic, U.K.
FEMS Microbiol Lett. 1990 Aug;58(3):249-54. doi: 10.1016/s0378-1097(05)80003-5.
Carbon monoxide dehydrogenase (CODH) from Pseudomonas thermocarboxydovorans strain C2 is composed of three non-identical subunits. A gene library of C2 DNA in lambda vector L47.1 was generated and screened using anti-CODH serum. Western blotting experiments revealed a protein which co-migrated with and had the same immunological reaction as the large subunit of CODH in some of the clones isolated from the library. The coding region was pinpointed to a 4 kb fragment which was subcloned into plasmid. Western blotting experiments showed that all three subunits of CODH were coded for by the subclone. However, no CODH activity was detected.
嗜热羧基营养假单胞菌C2菌株的一氧化碳脱氢酶（CODH）由三个不同的亚基组成。构建了C2 DNA在λ载体L47.1中的基因文库，并使用抗CODH血清进行筛选。蛋白质免疫印迹实验显示，在从文库中分离出的一些克隆中，有一种蛋白质与CODH的大亚基迁移率相同，且免疫反应相同。编码区域被定位到一个4 kb的片段，该片段被亚克隆到质粒中。蛋白质免疫印迹实验表明，CODH的所有三个亚基均由该亚克隆编码。然而，未检测到CODH活性。
